Multiple Choice Questions on Protein Synthesis

1. Protein synthesis refers to the
a) process of formation of amino acids directly from a DNA template
b) process of formation of mRNA from DNA template
c) process of formation of amino acids from mRNA
d) Process of duplicating DNA required for protein synthesis

transcription and translation

2. Which of the following is not a requirement for protein synthesis?
a) Ribosomes
b) peptidyl transfrase
c) Spliceosome
d)  amino acyl-tRNA synthetase

3. The enzyme involved in activation of serine is
a) peptidyl transfrase
b) amino acyl-tRNA synthetase
c) peptide polymerase
d) seryl-tRNA synthetase

4. Which is the source of energy for amino acid activation?
a) ATP
b) GTP
c) CTP
d) TTP

5. Which of the following statement is correct?
a) Termination codon has no tRNA
b) Activated amino acid binds to the 5’ end of respective tRNA molecule
c) CTP is required for amino acid activation
d)  there is only one amino acyl-tRNA synthetase in a cell

6. The preinitiation complex in prokaryotes include
a) Intiation factors, mRNA, 30 S subunit, 50 S subunit, ATP
b) Intiation factors, mRNA, 30 S subunit, GTP
c) Intiation factors, 30 S subunit, 50 S subunit, ATP
d) Intiation factors, mRNA, 50 S subunit, GTP

7. Peptide bond formation between amino acids of growing polypeptide chain is catalysed by
a) peptidyl transfrase
b) amino acyl-tRNA synthetase
c) peptide polymerase
d) Peptidyl synthetase

8. Translocation in protein synthesis begins with the
a) movement of tRNA from A-site to P-site
b) movement of dipeptidyl tRNA from A-site to P-site
c) movement of tRNA from P-site to A-site
d)  movement of tRNA from P-site to E-site

9. Peptidyl transfrase involved in peptide bond formation is located in the
a) 3’ region of tRNA
b) smaller subunit of ribosome
c) larger subunit of ribosome
d) Near Shine Dalgarno sequence


10. The amino acids are assembled into polypeptide chains on
a) mRNA
b) nucleus
c) ribosome
d) DNA template

11. Which of the following elongation factor is called as translocase?
a) EF2
b) EFG
c) both a and b
d) EF-Tu and EF-Ts

12. Which of the following release factor recognizes stop codons UGA and UAA?
a) Sigma factor
b) RF1
c) RF2
d) RF3
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Answers:
1. c) process of formation of amino acids from mRNA
2. c) Spliceosome
3. d) seryl-tRNA synthetase
4. a) ATP
5. a) Termination codon has no tRNA
6. b) Intiation factors, mRNA, 30 S subunit, GTP
7. a) peptidyl transfrase
8. b) movement of dipeptidyl tRNA from A-site to P-site
9. c) larger subunit of ribosome
10. c) ribosome
11. c) both a and b
12. c) RF2

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