Multiple Choice Questions on Monoclonal antibodies

1. Monoclonal antibodies are

a) heterogenous antibodies produced from single clone of plasma cells
b) homogenous antibodies produced from single clone of plasma cells
c) both a and b
d) none of these

Monoclonal and Polyclonal Antibodies

2. Natural humoral immune response against a pathogen leads to the production of

a) polyclonal antibodies
b) monoclonal antibodies
c) macrophages
d) none of these

3.The technology used for the production of monoclonal antibodies is

a) massculture technology
b) hybridoma technology
c) suspension culture
d) none of these

4. Hybridoma technology was developed by

a) Kohler and Milstein
b) Khorana and Nirenberg
c) Khorana and Korenberg
d) Beedle and Tautum

5. The hybridomas are made by

a) fusing T cells with myeloma cells
b) fusing B cells with myeloma cells
c) fusing T helper cells with myeloma cells
d) fusing B memory cells with myeloma cells

6. All are Mabs except

a) Rituximab
b) Transtuzumab
c) Infliximab
d) tamoxifen

7. Mabs are

a) specific towards a paratope
b) specific towards an epitope
c) specific towards an antigen
d) none of these

8. Which of the following statement is incorrect regarding HAT medium

a) HAT medium is a selective medium
b) Aminiopterin in the HAT medium blocks de novo pathway of nucleotide synthesis
c) Salvage pathway requires aminopterin and thymidine
d) Hypoxanthin is converted to guanine by HGPRT enzyme

9. HGPRT^- mutant cells are raised by inducing mutations using

a) 5-bromouracil
b) 8-azaguanine
c)  colchicine
d) 6-methy isocyanate

10. In hybridoma technology, hybrid cells are selected in

a) MS medium
b) HAT medium
c) x-gal medium
d) Whites medium

11. Which of the following cell is made deficient of hypoxanthine guanyl phosphoribosyl transferase (HGPRT) enzyme

a) B cells
b) hybrid cells
c) myeloma cells
d) none of these

12. Which of the following statement is incorrect regarding HAT selection

a) B cells are HGPRT + and can grow in HAT medium but undergoes normal cell death
b) Myeloma cells cannot grow in HAT medium as these cells lack HGPRT
c) Hybrid cell survive in HAT medium as it inherits HGPRT form B cells
d) Aminopterin in HAT medium blocks de novo pathway of nucleotide synthesis only in myeloma cells

13. Mabs are used in

a) the screening of recombinants
b) diagnostic kits
c) the treatment of many cancers
d) all of these

14. The major hazards of Mabs are

a) difficult in purification
b) contamination with retroviral particles from mouse myeloma cells
c) non specificity
d) all of these

15. Mabs are produced by

a) in vivo method
b) suspended cell culture in fermenters
c) Immobilized cell reactors
d) all of these

Answers

1. b) homogenous antibodies produced from single clone of plasma cells
2. a) polyclonal antibodies
3. b) hybridoma technology
4. a) Kohler and Milstein
5. b) fusing B cells with myeloma cells
6. d) tamoxifen
7. b) specific towards an epitope
8.c) Salvage pathway requires aminopterin and thymidine
9. b) 8-azaguanine
10. b) HAT medium
11. c) myeloma cells
12.d) Aminopterin in HAT medium blocks de novo pathway of nucleotide synthesis only in myeloma cells
13. d) all of these
14. b) contamination with retroviral particles from mouse myeloma cells
15.d) all of these