Multiple Choice Questions on Microtechnique Histology

1.Which of the following is an azodye
a) Safranin
b) Haematoxylin
c) Aniline blue
d) Orange G

2. Carnoy’s solution is a mixture of
a) Aqueous chromic acid, Aqueous acetic acid and distilled water
b) Ethyl alcohol, Glacial acetic acid and commercial formalin
c) Ethanol, Glacial acetic acid and Chloroform
d) Aqueous alcohol, Aqueous acetic acid and distilled water

3. The sectioning of woody materials for histo enzymological study is made
sledge microtome
a)Rotary microtome
b) Sledge microtome
c) Cryotome
d) Rocking microtome

4. Sudan Black B is often used for visualization of
a) Protein
b) Carbohydrates
c) Aminoacids
d) Lipids

5. Chromatography that involves the separation of isomers
a) Counter-current chromatography
b) Chiral chromatography
c) Paper chromatography
d) Thin layer chromatography

6. The total volume of material, both solid and liquid in the column is known as
a) Void volume
b) Bed volume
c) Elution volume
d) Retension volume

7. Silica gel-G is used in
a) Thin layer chromatography
b) Column chromatography
d) GLC

8. Histochemical localization of proteins is performed by using
a) Sudan black
b) Mercuric bromophenol blue
c) Periodic acid Schiffs (PAS) reagent
d) Iodine potassium iodide (IKI) solution

9. Orcein is obtained from
a) Fractionation of coal tar
b) Heartwood of Caesalpinia
c) Roccella tinctoria.
d) Abdominal part of insects Dactylopius coccus

10. Janus green B is used to stain
janus green B

a) Chloroplast
b) Golgi complex
c) Vacuoles
d) Mitochondria

1. c) Aniline blue
2. c) Ethanol, Glacial acetic acid and Chloroform
3. b) Sledge microtome
4. d) Lipids
5. b) Chiral chromatography
6. b) Bed volume
7. a) Thin layer chromatography
8. b) Mercuric bromophenol blue
9. c) Roccella tinctoria.
10. d) Mitochondria

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