MCQ on Immunological Techniques -RIA, ELISA, Immunoelectrophoresis | Immunology MCQ

1. Which among the following radioactive isotope is commonly used in Radioimmunoassay?

a) Sulphur-35

b) Iodine- 125

c) Phosphorus -32

d) Nitrogen -14

2. Blocking agent is used in western blotting. Identify the purpose

a) block the transfer of protein from gel to membrane

b) blocks the binding of antibody to the membrane

c) blocks the binding of primary and secondary antibody

d) blocks non-specific secondary antibody


3. Which among the following techniques makes use of immunofluorescence?

a) confocal microscopy

b) RIA


d) Electron microscopy

4. Identify the enzyme used in the technique of ELISA

a) Horse radish peroxidase 

b) Tyrosinase

c) Lactate dehydrogenase

d) Chymotrypsin 

5. Sandwich ELISA method is used for the detection of 

a) Antibody

b) Antigen

c) Conjugated antibody

d) Conjugated antigen 

6. Which membrane is commonly used in Northern blotting? 

a) Nitrocellulose 

b) Nylon

c) Polyvinylidenedifluoride membrane

d) None of these

7. SDS-PAGE is used in one of the following immunological technique?

a) Immunodiffusion

b) Western blotting 

c) Immunoelectrophoresis 

d) immunoprecipitation 

8. Western blotting is used for the detection of 

a) special glycolipid in a sample 

b) specific protein in a sample

c) specific DNA in a sample

d) specific RNA in a sample 

9. Match the following 

p. Immunodiffusion test       l. to measure antigen or antibody concentration 

q. Immuno-electrophoresis m. to separate several antigens

r. Radio immuno diffusion   n. to detect antigen or antibody in samples 

a) p-l, q-m, r-n

b) p-m. q-n, r-l

c) p-n, q-m, r-l

d) p-l, q-n, r-m

10. Which among the following will enable you to visualise a proteinaceous ligand binding to its cell surface receptor?


b) Polarised light microscopy 

c)Confocal microscopy with DAPI stain

d) Immunohistochemistry 

11. The formation of colored product at the end of ELISA technique is due to the addition of 

a) 4-chlorobutane

b) 4-chloromethane


d) none of the above 

12. In counter immunoeletrophoresis

a) the antibody will migrate towards anode

b) the antibody will migrate towards cathode

c)electrophoresis will drive the antibody and antigen parallel to each other.

d) electrophoresis will drive the antibody and antigen towards  to each other.

13. ELISA testing is used to diagnose which of the folllowing?

a) HIV infection

b) Blood typing


d) All of the above 


1. b) Iodine- 125

2. b) blocks the binding of antibody to the membrane

3. a) confocal microscopy

4. a) Horse radish peroxidase 

5. b) Antigen

6. b) Nylon

7. c) immunoelectrophoresis

8. b) specific protein in a sample

9. c) p-n, q-m, r-l

10. d) Immunohistochemistry

11. c)4-chloronapthol

12. d) electrophoresis will drive the antibody and antigen towards  to each other.

13. d) All of the above 

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