1. Which among the following radioactive isotope is commonly used in
Radioimmunoassay?
a) Sulphur-35
b) Iodine- 125
c) Phosphorus -32
d) Nitrogen -14
2. Blocking agent is used in western blotting. Identify the purpose
a) block the transfer of protein from gel to membrane
b) blocks the binding of antibody to the
membrane
c) blocks the binding of primary and secondary antibody
d) blocks non-specific secondary
antibody
3. Which among the following techniques makes use of immunofluorescence?
a) confocal microscopy
b) RIA
c) ELISA
d) Electron microscopy
4. Identify the enzyme used in the technique of ELISA
a) Horse radish peroxidase
b) Tyrosinase
c) Lactate dehydrogenase
d) Chymotrypsin
5. Sandwich ELISA method is used for the detection of
a) Antibody
b) Antigen
c) Conjugated antibody
d) Conjugated antigen
6. Which membrane is commonly used in Northern blotting?
a) Nitrocellulose
b) Nylon
c) Polyvinylidenedifluoride membrane
d) None of these
7. SDS-PAGE is used in one of the following immunological technique?
a) Immunodiffusion
b) Western blotting
c) Immunoelectrophoresis
d) immunoprecipitation
8. Western blotting is used for the detection of
a) special glycolipid in a sample
b) specific protein in a sample
c) specific DNA in a sample
d) specific RNA in a sample
9. Match the following
p. Immunodiffusion test
l. to measure antigen or antibody concentration
q. Immuno-electrophoresis m. to
separate several antigens
r. Radio immuno diffusion
n. to detect antigen or antibody in samples
a) p-l, q-m, r-n
b) p-m. q-n, r-l
c) p-n,
q-m, r-l
d) p-l, q-n, r-m
10. Which among the following will
enable you to visualise a proteinaceous ligand binding to its cell surface
receptor?
a) ELISA
b) Polarised light microscopy
c)Confocal microscopy with DAPI stain
d) Immunohistochemistry
11. The formation of colored product at the end of ELISA technique is due to the addition of
a) 4-chlorobutane
b) 4-chloromethane
c)4-chloronapthol
d) none of the above
12. In counter immunoeletrophoresis
a) the antibody will migrate towards anode
b) the antibody will migrate towards cathode
c)electrophoresis will drive the antibody and antigen parallel to each other.
d) electrophoresis will drive the antibody and antigen towards to each other.
13. ELISA testing is used to diagnose which of the folllowing?
a) HIV infection
b) Blood typing
c)Pregnancy
d) All of the above
Answers:
1. b) Iodine- 125
2. b) blocks the binding
of antibody to the membrane
3. a) confocal
microscopy
4. a) Horse radish
peroxidase
5. b) Antigen
6. b) Nylon
7. c) immunoelectrophoresis
8. b) specific protein
in a sample
9. c) p-n, q-m, r-l
10. d) Immunohistochemistry
11. c)4-chloronapthol
12. d) electrophoresis will drive the antibody and antigen towards to each other.
13. d) All of the above
