Multiple Choice Questions on Molecular Biology Techniques

1. The technique used to identify specific DNA sequence in bacterial colonies is

a) colony hybridization
b) in situ hybridization
c) dot blot technique
d) western blotting

2. Sothern hybridization is

a) used to identify a specific protein
b) used to identify a specific DNA
c) used to identify a specific RNA
d) used to identify both DNA and RNA

3. Which of the following statements are true regarding southern blotting

a) developed by E.M.Southern
b) DNA-DNA hybridization is the basis
c) The transfer of DNA fragments from gel to nitrocellulose membrane is called blotting
d) all of these

4. Applications of Southern blotting includes

a) DNA fingerprinting
b) preparation of RFLP maps
c) identification of transferred genes
d) all of these

5. The technique used to detect the presence of DNA or RNA in a non-fractionated DNA sample is

a) colony hybridization
b) in situ hybridization
c) dot blot technique
d) western blotting

6. Northern hybridization is

a) used to identify a specific protein
b) used to identify a specific DNA
c) used to identify a specific RNA
d) used to identify both DNA and RNA

7. In Northern hybridization probe hybridization forms

a) DNA:DNA hybrid
b) RNA:DNA hybrid
c) both a and b
d) none of these

8. All are differences in procedure between Northern and Southern hybridization except

a) DBM membrane is used in northern hybridization
b) RNA:DNA hybrids are formed in northern hybridization
c) Intially fragments are separated by electrophoresis in northern hybridization
d) DNA denaturation is required before blotting in Sothern hybridization

9. Sothern hybridization is

a) used to identify a specific protein
b) used to identify a specific DNA
c) used to identify a specific RNA
d) used to identify both DNA and RNA

10. In Western blotting

a) agarose gel is commonly used
b) polyacrylamide gel is commonly used
c) both a and b
d) high resolution gels

11. For glycoproteins, most commonly used probe is

a) antibody
b) lectin
c) antigens
d) interferons

12. For protein detection, most commonly used probe is

a) antibody
b) lectin
c) antigens
d) interferons

Elisa plate

13. ELISA is based on

a) antigen-antibody interaction
b) antigen-protein intercation
c) lectin- antibody interaction
d) all of these

14. The technique used to locate specific genes in chromosomes is

a) colony hybridization
b) in situ hybridization
c) dot blot technique
d) western blotting

15. The separation technique of charged molecules under the influence of electric current is called

a) colony hybridization
b) electrophoresis
c) dot blot technique
d) western blotting

 

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Answers:
1. a) colony hybridization
2. b) used to identify a specific DNA
3. d) all of these
4. d) all of these
5. c) dot blot technique
6. c) used to identify a specific RNA
7. c) both a and b
8. c) Initially fragments are separated by electrophoresis in northern hybridization
9. a) used to identify a specific protein
10. b) polyacrylamide gel is commonly used
11. b) lectin
12. a) antibody
13. a) antigen-antibody interaction
14. b) in situ hybridization
15. b) electrophoresis