Multiple Choice Questions on Molecular Markers

1. Specific biomolecules which show easily detectable differences among different strains of a species or among different species is termed as

a) DNA fingerprinting
b) molecular markers
c) molecular scissors
d) RFLP

2. Molecular markers include

a) RFLP
b) RAPD
c) AFLP
d) all of these

3. Molecular markers are used to construct

a) chromosome maps
b) cytogenetic maps
c) physical maps
d) all of these

4. The variation in the restriction DNA fragment lengths between individuals of a species is called

RFLP

a) restriction Fragment Length Polymorphism (RFLP)
b) Random amplified Polymorphic DNA (RAPD)
c) Amplified Fragment Length Polymorphism (AFLP)
d) Simple Sequence repeats (SSR)

5. RFLP is used to

a) construct high resolution linkage maps
b) identify single gene diseases
c) construct QTL maps
d) all of these

6. RFLP involves

a) used to identify a specific protein
b) used to identify a specific DNA
c) used to identify a specific RNA
d) used to identify both DNA and RNA

7. Locations of quantitative genes on chromosomes are called

a) Qualitative trait loci
b) Quatitative trait loci
c) both a and b
d) none of these

8. RAPD is a

a) DNA sequencing based method
b) Restriction digestion based method
c) PCR based method
d) all of these

9. The set of DNAs generated by using random primers in a PCR reaction is called

a) RAPD
b) RFLP
c) AFLP
d) in situ hybridization

10. All the statements are true regarding RFLP and RAPD except

a) RAPD is a quick method compared to RFLP
b) RFLP is more reliable than RAPD
c) Species specific primers are required for RAPD
d) Radioactive probes are not required in RAPD

11. DNA of eukaryotic organisms has several repeating units of short sequences called

a) random repeats
b) tandem repeats
c) mini satellites
d) all of these

12. The variation in number of tandem repeats between two or more individuals is called

a) Variable number of tandem repeats (VNTRs)
b) Restriction Fragment Length Polymorphism (RFLP)
c) Simple sequence repeats (SSRs)
d) Amplified Fragment Length Polymorphism (AFLP)

13. Simple sequence repeats are

a) 1-6 bp long sequences distributed along the chromosome
b) also called as micro satellites
c) individual specific in number and position
d) all of these

14. AFLP is a

a) PCR based method
b) method to detect polymorphism in the DNA throughout the genome
c) method that detects the presence or absence of a fragment
d) all of these

15. The variant fragment that distinguish one individual from another one is called

a) variant fragment
b) marking fragment
c) differing fragment
d) all of these

Answers
1. b) molecular markers
2. d) all of these
3. d) all of these
4. a) restriction Fragment Length Polymorphism (RFLP)
5. d) all of these
6. c) used to identify a specific RNA
7. b) Quatitative trait loci
8. c) PCR based method
9. a) RAPD
10. c) Species specific primers are required for RAPD
11. b) tandem repeats
12. a) Variable number of tandem repeats (VNTRs)
13. d) all of these
14. d) all of these
15. b) marking fragment