1. A recombinant DNA molecule is produced by
a) joining of two DNA fragmentsb) joining of two or more DNA fragmentsc) both a and bd) joining of two or more DNA fragments originating from different organisms2. The gene formed by the joining of DNA segments from two different sources are called asa) recombinant geneb) joined genec) both a and bd) chimaeric gene3. Which of the following enzyme is used to cut DNA molecule in rDNA technologya) ligaseb) phosphatasec) ribonucleased) restriction enzymes4. Restriction enzymes are also called asa) biological scissorsb) molecular scalpelsc) molecular knivesd) all of these5. The most important discovery that lead to the development of rDNA technology wasa) Double helix model of Watson and Crickb) discovery of restriction enzymesc) discovery of ligase enzymed) discovery of plasmids6. Who discovered restriction enzymesa) Nathan, Arber and Smith in 1970b) Watson, Crick and Wilkins in 1970c) Boyer and Cohen in 1975d) Paul Berg in 19757. Who created the first rDNA moleculea) Nathan, Arber and Smithb) Watson, Crick and Wilkinsc) Boyer and Cohend) Paul Berg8. The DNA molecule to which the gene of insert is integrated for cloning is calleda) carrierb) transformerc) vectord) none of these9. The DNA segment to be cloned is calleda) gene segmentb) DNA fragmentc) DNA insertd) all of these10. Which of the following statements are true regarding rDNA technologya) rDNA technology is used to obtain large number of copies of specific DNA fragmentsb) rDNA technology is used to obtain large quantities of the protein produced by the concerned genec) rDNA technology is used to integrate gene of interest into chromosomes where it expresses itselfd) all of these11. The first successful transformation of rDNA molecule into a bacterium was carried out bya) Nathan, Arber and Smithb) Watson, Crick and Wilkinsc) Boyer and Cohend) Paul Berg12. The plasmid used by Cohen and Boyer for their transformation experiment wasa) pSC 101b) PUC 17c) pBR 322d) E.coli plasmids13. The mechanism of intake of DNA fragments from the surrounding medium by a cell is calleda) transformationb) transductionc) both a and bd) conjugation14. Gene cloning refers to thea) production of large number of copies of the gene being clonedb) production of asexual progeny from a single individual or a cellc) both a and bd) none of these15. Paul Berg’s gene splicing experiment created the first rDNA molecule which was aa) a T4 phage fragment incorporated into SV40 vectorb) a lambda phage fragment incorporated into SV40 vectorc) a T4 phage fragment incorporated into pSC 101 vectord) a lambda phage fragment incorporated into pSC 101 vector
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Answers:1. d) joining of two or more DNA fragments originating from different organisms2. d) chimaeric gene3. d) restriction enzymes4. a) biological scissors5. b) discovery of restriction enzymes6. a) Nathan, Arber and Smith in 19707. d) Paul Berg8. c) vector9. c) DNA insert10. d) all of these11. c) Boyer and Cohen12. a) pSC 10113. a) transformation14. a) production of large number of copies of the gene being cloned15. b) a lambda phage fragment incorporated into SV40 vector
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