1. The technique electrophoresis, for the separation of charged
molecules was developed by
a) Tswett
b) Svedberg
c) Tiselius
d) Sanger
2. In electrophoresis, DNA will migrate towards
a) cathode or positive electrode
b) anode or negative electrode
c) cathode or negative electrode
d) anode or positive electrode
3. The speed of migration of ions in an electric field depends
on
a) magnitude of charge and mass of molecules
b) magnitude of charge and shape of molecules
c) shape and size of the
molecule
d) magnitude of charge, shape and mass of molecules
4. Which of the following statement is true regarding migration
of bio molecules?
a) the rate of migration is directly proportional to the current
b) the rate of migration is inversely proportional to current
c) the rate of migration is directly proportional to the
resistance of the medium
d) Low voltage is used for the separation of high molecular
weight compounds
5. Electrophoresis cell or electrophoresis apparatus consists of
a) power pack and electrophoresis unit
b) Elctrophoresis unit and DNA separator
c) buffer chamber and Elctrophoresis unit
d) Gel, buffer chamber and power pack
6. The most common type of gel used for DNA separation is
a) Agar
b) Polyacrylamide
c) Agarose
d) All of the above
7. Which is the technique suited for the separation of large DNA
fragments
a) AGE
b) PAGE
c) PFGE
d) SDS-PAGE
8. What is the role of SDS in SDS-PAGE?
a) protein denaturing and imparting net negative charge
b) imparting overall negative charge to the protein
c) imparting equal mass to all proteins
d) protein unfolding and
imparting net positive charge
9. In SDS-PAGE, separation is based on
a) molecular weight
b) shape
c) charge
d) all of the above
10. The electrophoresis technique that used isoelectric focusing
is
a) AGE
b) PFGE
c) 2D-PAGE
d) SDS-PAGE
11. If proteins are separated according to their electrophoretic mobility then the type of electrophoresis is
a) SDS-PAGE
b) PFGE
c) Electro focusing
d) Affinity electrophoresis
12.The Polymerisation of gel used in PAGE occurs between polyacrylamide and ----
a) bisacrylamide
b) N, N-methylene bisacrylamide
c) N, N-Acrylamide
d) PEG
13. SDS used in PAGE is--
a) cationic detergent
b) an anion exchanger
c) an anionic detergent
d) An non ionic detergent
14. Role of APS in SDS PAGE is to:
a) act as a catalyst in the polymerization of acrylamide
b) act as pore builder in the polymerized gel
c) act as source of free radicals
d) None of these
15. The tracking dye used in SDS PAGE will be
a) cationic
b) amphipathic
c) an anionic
d) non -ionic
*AGE: Agarose Gel Electrophoresis, PAGE: Polyacrylamide Gel
Electrophoresis, PFGE: Pulse Field Gel Electrophoresis, SDS: sodium dodecyl
sulphate
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Answers:
1. c) Tiselius
2. d) anode or positive electrode
3. b) magnitude of charge and shape of molecules
4. a) the rate of
migration is directly proportional to current
5. a) power pack and electrophoresis unit
6. c) Agarose
7. c) PFGE
8. a) protein denaturing and imparting net negative charge
9. a) molecular weight
10. c) 2D-PAGE
11. a) SDS-PAGE
12. b) N, N-methylene bisacrylamide
13. c) an anionic detergent
14. c) act as source of free radicals
15. c) an anionic
Tags:
Biotechnology MCQs
MCQ on Electrophoresis
molecular biology mcq
Molecular Biology Techniques
Molecular Biology Techniques mcq
Multiple choice on Electrophoresis
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