MCQ on Animal Cell Culture

 

1. Viability of cells in animal cell culture can be determined by:

1. Bradford's assay 2. Trypan Blue assay 3. Comet assay 4. MTT assay

a) 2 only 

b) 1 & 2 only 

c) 2 & 4 only 

d) 1 & 3 only 

 

2. In animal cell culture the CO2 levels in the incubators are usually maintained at:

a) 2 % 

b) 1 % 

c) 5 % 

d) 10 % 

 

3. The pH indicator in animal cell culture medium is: 

a) HEPES 

b) Phenol Red 

c) FBS 

d) L-Glutamine

 

4. Which of the following is an anchorage-independent cell line? 

A) MCF7 

B) MDA MB 231 

C) K562 

D) PC-3 

 

5. Heat inactivation of Foetal Bovine Serum is done at 

 A) 560C for 30 min

 B) 370for 60 min 

 C) 56 0for 10 min 

D) 370for 15 min  

 

6. Application of gene therapy in clinical trials did NOT succeed due to 

a) poor integration of a gene in the host genome

b) lack of expression of the a integrated gene in cells

c) degradation of gene inside the cell

d) activation of oncogenes consequent to the integration of the gene

MCQ on Animal Cell Culture

 

7. For sustainable expression of a transgene in the successive generation of a cell line in culture, the ideal gene transfer can be obtained using 

a) lentiviral vector

b) adenoviral vector

c) plasmid DNA containing the transgene

d) only transgenic DNA

 

8. Which of the following host systems is best suited to express large amounts of glycosylated protein for structural studies?

a) GST fusion protein in E.coli

b) His-tagged protein in E.coli

c) Native protein in baculovirus

d) Native protein in Pseudomonas fluorescens

 

9. For developing transgenic mice, embryonic stem cells are engineered to express the transgene. These cells are  selected by 

a) novobiocin.

b) neomycin

c) tetracycline. 

d) penicillin

 

10. To keep them in a totipotent state, embryonic stem cells  need to be maintained in a medium supplemented with

a) growth hormone 

b) leukemia inhibiting factor

c) nestin

d) insulin

 

11. Which one of the following techniques is generally used to produce transgenic animals? 

a) Processed mRNA containing only exons are introduced into the embryo.

b) Entire foreign nucleus is introduced in the blastocyst-stage   enucleated unfertilized egg

c) Desired DNA is microinjected into fertilized eggs followed by implantation of the embryo in a foster mother.

d) c-DNA of the desired gene is introduced into animal embryos and implanted in a foster mother

 

12. Which one of the following statements is NOT correct for propagation and maintenance of mammalian cells in vitro?

a) Transformed cell lines do need  an external supply of serum need an to grow.

b) The cells that are obtained directly from the organism is a primary culture.

c) Trypsin is added to cell culture media to maintain cell's health.

d) HEPES buffer is generally used to maintain pH of the culture media.

 

13. To prepare individual tissue cells from a primary culture, the cell-cell and cell-matrix interaction must be broken. To achieve this would NOT use:

a) EDTA

b) Trypsin

c) Collagenase

d) Separase

 

14. Which is best method for checking mycoplasma contamination in a mammalian cell line? 

a) Southern Hybridization

b) ELISA

c) PCR

d) Western Hybridization

 

Learn more: MCQ on Animal Biotechnology

Answers

1. c) 2 & 4 only 

2. c) 5 % 

3. b) Phenol Red

4.c) K562 

5.  A) 560C for 30 min

6. d) activation of oncogenes consequent to the integration of the gene

7. a) lentiviral vector

8. c) Native protein in baculovirus

9. b) neomycin

10. b) leukemia inhibiting factor

11. c) Desired DNA is microinjected into fertilized eggs followed by implantation of embryo in a foster mother.

12. c) Trypsin is added to cell culture media to maintain cell's health.

13. d) Separase

14. c) PCR

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