1. Viability of cells in animal cell culture can be determined by:
1. Bradford's assay 2. Trypan Blue assay 3. Comet assay 4. MTT assay
a) 2 only
b) 1 & 2 only
c) 2 & 4 only
d) 1 & 3 only
2. In animal cell culture the CO2 levels in the incubators are usually maintained at:
a) 2 %
b) 1 %
c) 5 %
d) 10 %
3. The pH indicator in animal cell culture medium is:
a) HEPES
b) Phenol Red
c) FBS
d) L-Glutamine
4. Which of the following is an anchorage-independent cell line?
A) MCF7
B) MDA MB 231
C) K562
D) PC-3
5. Heat inactivation of Foetal Bovine Serum is done at
A) 560C for 30 min
B) 370C for 60 min
C) 56 0C for 10 min
D) 370C for 15 min
6. Application of gene therapy in clinical trials did NOT succeed due to
a) poor integration of a gene in the host genome
b) lack of expression of the a integrated gene in cells
c) degradation of gene inside the cell
d) activation of oncogenes consequent to the integration of the gene
7. For sustainable expression of a transgene in the
successive generation of a cell line in culture, the ideal gene transfer can be
obtained using
a) lentiviral vector
b) adenoviral vector
c) plasmid DNA containing the transgene
d) only transgenic DNA
8. Which of the following host systems is best suited
to express large amounts of glycosylated protein for structural studies?
a) GST fusion protein in E.coli
b) His-tagged protein in E.coli
c) Native protein in baculovirus
d) Native protein in Pseudomonas fluorescens
9. For developing transgenic mice, embryonic stem
cells are engineered to express the transgene. These cells are selected
by
a) novobiocin.
b) neomycin
c) tetracycline.
d) penicillin
10. To keep them in a totipotent state, embryonic stem
cells need to be maintained in a medium supplemented with
a) growth hormone
b) leukemia inhibiting factor
c) nestin
d) insulin
11. Which one of the following techniques is generally
used to produce transgenic animals?
a) Processed mRNA containing only exons are introduced
into the embryo.
b) Entire foreign nucleus is introduced in the
blastocyst-stage enucleated unfertilized egg
c) Desired DNA is microinjected into fertilized eggs
followed by implantation of the embryo in a foster mother.
d) c-DNA of the desired gene is introduced into animal
embryos and implanted in a foster mother
12. Which one of the following statements is NOT
correct for propagation and maintenance of mammalian cells in vitro?
a) Transformed cell lines do need an external
supply of serum need an to grow.
b) The cells that are obtained directly from the
organism is a primary culture.
c) Trypsin is added to cell culture media to maintain
cell's health.
d) HEPES buffer is generally used to maintain pH of
the culture media.
13. To prepare individual tissue cells from a primary
culture, the cell-cell and cell-matrix interaction must be broken. To achieve
this would NOT use:
a) EDTA
b) Trypsin
c) Collagenase
d) Separase
14. Which is best method for checking mycoplasma
contamination in a mammalian cell line?
a) Southern Hybridization
b) ELISA
c) PCR
d) Western Hybridization
15. An animal viral vector:
a) CMV
b) TMV
c) SV40
d) Gemini
Learn more: MCQ on Animal Biotechnology
Answers
1. c) 2 & 4 only
2. c) 5 %
3. b) Phenol Red
4.c) K562
5. A) 560C for 30 min
6. d) activation of oncogenes consequent to the
integration of the gene
7. a) lentiviral vector
8. c) Native protein in baculovirus
9. b) neomycin
10. b) leukemia inhibiting factor
11. c) Desired DNA is microinjected into
fertilized eggs followed by implantation of embryo in a foster mother.
12. c) Trypsin is added to cell culture media to
maintain cell's health.
13. d) Separase
14. c) PCR
15. c) SV40
