1. The cDNA library of the organism will be
A)
Tissue-specific
B)
Growth specific
C)
Organ-specific
D)
Cell-specific
2. Eco R1 is the restriction enzyme that recognizes the following DNA sequence
A)
GGATCC
CCTAGG
B)
GAATTC
CTTAAG
C)
GCGC
CGCG
D)
TCGA
AGCT
3. Axenic culture is best defined as
A)
Cell culture
B)
Organ culture
C)
Cell culture of microbes
D)
Cell culture free from other microbes
4. An analysis of chromosomal DNA by Southern blot technique, involves the following
steps
1.
Autoradiography 2. Blotting 3. Cleavage 4. Electrophoresis 5. Hybridization
Which
of the following sequence of steps best illustrates this technique?
A)
1,2,3,4,5
B)
1,3,2,4,5
C)
3,5,2,4,1
D)
3,4,2,5,1
5.
Bacterial artificial chromosome, cosmid, P1phage and plasmid are
common cloning vectors with a range from 1 to 300k. Which of the following
is the proper order for these vectors in terms of cloning capacity?
A)
Bacterial artificial chromosome, cosmid, P1 phage, plasmid
B)
Cosmid, Bacterial artificial chromosome, P1 phage, plasmid
C) P1
Phage, cosmid, Bacterial artificial chromosome, plasmid
D)
Plasmid, cosmid, P1 phage, Bacterial artificial chromosome
6. The DNA sequence cut by Eco RI is
A)
GCAT /GCAT
B)
GAATTC/GAATTC
C)
GAATTC/CTTAAG
D)
GAATTG/CTTTAAC
7.
Any one of a group of restriction enzymes that recognizes the same
nucleotide sequences
A)
Isozyme
B)
Ribozyme
C)
Isoschizomer
D)
Isomer
8. In prenatal diagnosis, the polymorphism used to determine genetic
disorders is
A)
SNPs
B)
RFLPs
C)
RAPDs
D)
SCARs
9. A
Drug resistance marker used for the selection of recombinants is
A)
NPT II
B)
SSP
C)
SSR
D) AFLP
10.
Which of the following statements are true about R-Plasmids?
I. It
is the extrachromosomal genetic material
II.
It has genes encoding bacterial toxins
III.
It is circular and confer resistance to antibiotics
IV.
It can spread fast in bacterial populations
A) I
and II
B)I
and III
C)II
and III
D) I, III and IV
11. You
have been tasked with cloning a plant gene in bacterial systems and later its
expression in cultured cells. Which type of vector you should select for easy
cloning and expression?
A)
Phage Vector
B)
Cosmid Vector
C)
Shuttle Vector
D)
YAC
12. Green Fluorescent Protein is isolated from:
A)
Photimus pyralis B) Aequorea Victoria C) Pyrococcus furiosus
D) Thermus aquaticus
Learn more: Topic-wise Biotechnology MCQ
Answers:
1. A) Tissue-specific
2. B) GAATTC
CTTAAG
3. D) Cell culture free from other microbes
4. D) 3,4,2,5,1
5. D) Plasmid, cosmid, P1 phage, Bacterial artificial chromosome
6. C) GAATTC/CTTAAG
7. C) Isoschizomer
8. A) SNPs
9. A) NPT II
10.D) I, III, and IV
11. C) Shuttle Vector
12. B) Aequorea Victoria
