Staining Techniques - Gram Staining, Acid Fast Staining, Endospore Staining | Techniques in Microbiology MCQs

Staining Techniques - Gram Staining, Acid Fast Staining, Endospore Staining | Techniques in Microbiology MCQs


1. If only one stain is used for staining a specimen

A) Simple staining
B) Negative staining

C) Differential staining 

D) None of these

 

2. Other than the sample (specimen) the remaining portion is stained then it is called

A) Simple staining

B) Negative staining

C) Differential staining

D) None of these

 

3. If more than one stain is used, such staining is called

A) Simple staining

B) Negative staining

C) Differential staining

D) None of these

 

4. Gram staining is an example for

A) Simple staining

B) Differential staining

C) Negative staining

D) None of these

5. The order of stains in Gram-staining procedure is

A) Crystal violet, Iodine solution, Alcohol, Saffranine

B) Iodine solution, Crystal Violet, Saffranine, Alcohol

C) Alcohol, Crystal Violet, Iodine solution, Saffranine

D) None of these

 

6. The percentage of alcohol used in Gram staining is

A) 75% B) 90% C) 60% D) 25%

 

7. Gram positive bacteria appear as

A) Pink B) Violet C) both a & b D) None of these

 

8. Gram negative bacteria appear as

A) Pink B) Violet C) both a & b D) None of these

 

9. The action of alcohol during Gram staining is

A) Allows the color

B) It adds color

C) Decolorises the cells

D) None of these

 

10. Staining material of Gram-positive bacterium is

A) Fast green

B) Haematoxylon

C) Crystal violet

D) Safranin

 

11. The staining technique used to stain the metachromatic granules of Corynebacterium

A) Giemsa stain

B) Alberts stain

C) Acid fast staining

D) Both a and b

 

12. The procedure of differential staining of bacteria was developed by

A) A H. Gram

B) H.C Gram

C) N.C Gram

D) H.A Gram

 

13. Mordant used in Grams staining is

A) Crystal violet

B) Iodine

C) Saffranin

D) All of these

 

 

14. Chlamydiae can be stained better with

A) Ziehl neelsen staining

B) Castaneda & Machiavello stains

C) Giminez stains

D) Both B and C

 

15. During staining for Electron Microscopy, the method which improves contrast of specimen is

A) Positive staining

B) Negative staining

C) Shadow staining

D) None of these

 

16. Which of the staining technique helps in demonstrating spore structure in bacteria as well as free spores?

A) Acid-fast stain

B) Endospore stain

C) Capsule stain

D) Flagella stain

17. Mycobacteria are stained with

A) Gram’s staining

B) Simple staining

C) Both a and b

D) Ziehl – Neelsen’s staining

 

18. Differential staining of bacteria spore is related to

A) Albert’s staining

B) Lugol’ s staining

C) Moller’s staining

D) Indian ink preparation

 Learn more: Staining Techniques - Gram stain, Acid Fast Satin, Endospore Stain | Techniques in Microbiology

Answers:

1-A

2-B

3-C

4-D

5-A

6-B

7-B

8-A

9-C

10-C

11-B

12-B

13-B

14-D

15-B

16-B

17-D

18-C

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