MCQ on CRISPR-Cas9 Technology
1. Who were the two scientists who won the 2020 Nobel Prize
in Chemistry for their work on CRISPR-Cas9?
(A) Emmanuelle Charpentier and Jennifer Doudna
(B) Francis Collins and Craig Venter
(C) George Church and Feng Zhang
(D) Jack Szostak and Elizabeth
Blackburn
2. What does CRISPR-Cas9 stand for?
(A) Cas 9 Regulated Interspaced
Short Palindromic Repeats
(B) Clustered Regularly
Interspaced Small Palindromic Repeats-Cleaved Sequence 9
(C) Clustered Regularly
Interspaced Short Palindromic Repeats-Caspase 9
(D) Clustered Regularly
Interspaced Short Palindromic Repeats-Cas9
3. What are the two main components of CRISPR-Cas9?
(A) CRISPR RNA and Cas1 protein
(B) Guide RNA and Cas9 protein
(C) Guide RNA and Cas2 protein
(D) CRISPR RNA and Cas9 Nuclease
4. How does CRISPR-Cas9 work?
(A) The guide RNA binds to a
specific DNA sequence, and the Cas9 protein cuts the DNA at that location.
(B) The CRISPR RNA binds to a
specific DNA sequence, and the Cas9 protein repairs the DNA at that location.
(C) The guide RNA binds to a random
DNA sequence, and the Cas9 protein deletes the DNA at that location.
(D) The guide RNA binds to a
specific DNA sequence, and the Cas9 protein inserts a new DNA sequence at that
location.
5. What are some of the potential applications of CRISPR-Cas9?
(A) Gene therapy
(B) Crop improvement
(C) Disease treatment
(D) All of the above
6. What are some of the ethical concerns associated with CRISPR-Cas9?
(A) The potential for creating
designer babies
(B) The potential for accidental
gene editing
(C) Ecological imbalance
(D) All of the above
7. What are some of the limitations of CRISPR-Cas9?
(A) It is not always possible to
target the desired DNA sequence.
(B) It can be difficult to
control the extent of gene editing.
(C) It can cause non targeted
mutations.
(D) All of the above
8. Cas9 is an
(A) exonuclease that makes ds
breaks in DNA
(B) exonuclease that makes ss
breaks in RNA
(C) endonuclease that makes ds
breaks in DNA
(D) endonuclease that makes breaks
in DNA and RNA
9. CRISPR-Cas9 was originally discovered as a part of defense mechanism against viruses in
(A) Fungi
(B) Bacteria
(C) Humans
(D) Chimps
10. Single stranded RNA that is complementary to the target DNA
sequence used in CRISPR-Cas9 is called
(A) snoRNA
(B) guide RNA
(C) Cas RNA
(D) spliceosome
11. Which of the following statements
is true regarding DNA repair in CRISPR-Cas9 gene editing?
(A) Endogenous DNA repair to
repair double-strand breaks by Cas9
(B) DNA repair by nonhomologous
end-joining (NHEJ)
(C) DNA repair by homology-directed
repair (HDR)
(D) All of the above
12. Which of the following is not
a genome editing technology?
(A) DNA foot printing
(B) Zinc finger nuclease (ZFN)
(C) Transcription activator-like
effector nuclease (TALEN)
(D) CRISPR-Cas9
Watch our summary video on the topic!
Answers
1. (A) Emmanuelle Charpentier and
Jennifer Doudna
2. (D) Clustered Regularly
Interspaced Short Palindromic Repeats-Cas9
3. (B) Guide RNA and Cas9 protein
4. (A) The guide RNA binds
to a specific DNA sequence, and the Cas9 protein cuts the DNA at that location.
5. (D) All of the above
6. (D) All of the above
7. (D) All of the above
8. (C) endonuclease that makes ds
breaks in DNA
9. (B) Bacteria
10. (B) guide RNA
11. (D) All of the above
12. (A) DNA foot printing
Understand More: 5 Steps in CRISPR-Cas9 Technology
