Multiple Choice Question on DNA Sequencing || Molecular Biology Techniques MCQ

1. DNA sequencing refers to the
a) technique used to determine the sugar sequence in a DNA molecule
b) technique used to determine the phosphate sequence in a DNA molecule
c) technique used to determine the base sequence in a DNA molecule
d) All of these

2. Which of the following is a chemical nucleotide sequencing method?
a) Sanger method
b) Maxam-Gilbert method
c) Edmans method
d) Automated sequencing method
3. The enzyme used in Maxam-Gilbert method for ³²P labelling of the DNA at 3’ end is
a) polynucleotide kinase
b) alkaline phosphatase
c) exonuclease
d) terminal nucleotidyl transferase
4. How many different types of chemical treatments are required in Maxam-Gilbert method?
a) 1
b) 2
c) 3
d) 4 
5. Guanine specific cleavage in Maxam-Gilbert method is done by using
a) formic acid
b) hydrazine
c) Dimethyl sulphate
d) piperidine
6. The principle of Sanger’s method relies on
a) Use of chemicals for base specific cleavage
b) Use of dNTPs for chain termination
c) Use of ddNTPs for chain termination
d) Use of for ³²P chain termination

7. The samples in Sanger’s method after reaction are separated using
a) AGE
b) PAGE
c) PFGE
d) 2-D gel electrophoresis 
8. Which of the following sequencing methods uses PCR for generating sequence templates?
a) Sanger’s method
b) Sanger’s method and LMPCR
c) LMPCR
d) LMPCR and automated DNA sequencing
9. Automated DNA sequencing is an improvement of Sanger’s method where
a) ddNTPS are used for chain termination
b) PCR is used for making sequencing templates
c) Fluorescently labelled dNTPs are used for chain termination
d) Fluorescently labelled ddNTPs are used for chain termination
10. Which of the following is not a DNA sequencing method?
a) LMPCR
b) Edmans method
c) Sanger’s method
d) Maxam-Gilbert method
Learn more: MCQ on DNA fingerprinting

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Answers:
1. c) technique used to determine the base sequence in a DNA molecule
2. b) Maxam-Gilbert method
3. a) polynucleotide kinase
4. d) 4 
5. c) Dimethyl sulphate
6. c) Use of ddNTPs for chain termination
7. b) PAGE
8. c) LMPCR
9. d) Fluorescently labelled ddNTPs are used for chain termination
10. b) Edman’s method