Multiple Choice Questions on Transcription

1. The process involved in the RNA formation on the DNA template is
Eukaryotic transcription

a) Transcription
b) Translation
c) Replication
d) Transformation
2. The enzyme required for transcription is
a) Restriction enzymes
b) DNA polymerase
c) RNA polymerase
d) RNAase
3. Transcription is the transfer of genetic information from
a) DNA to RNA
b) tRNA to mRNA
c) DNA to mRNA
d) mRNA to tRNA
4. RNA required for the protein synthesis
a) mRNA
b) tRNA
c) rRNA
d) All of these
5. A promoter site on DNA
Promoter site
a) Initiates transcription
b) Regulates termination
c) Codes for RNA
d) Transcribes repressor
6. Sigma factor is component of
a) DNA ligase
b) DNA polymerase
c) RNA polymerase
d) endonuclease
7. What is the main function of tRNA in relation to protein synthesis?
a) Inhibits protein synthesis
b) Proof reading
c) Identifies amino acids and transport them to ribosomes
d) all of these

8. RNA polymerase (Ochoa 1953) has polypeptide chains
RNA polymerase

a) 2
b) 3
c) 4
d) 5
9. Which site of tRNA molecule hydrogen bonds to a mRNA molecule?
tertiary structure of tRNA

a) Codon
b) Anticodon
c) 5’ends of the tRNA molecule
d) 3’ends of the tRNA molecule
10. The DNA chain acting as a template for RNA synthesis has the following order of bases, AGCTTCGA. What will be the order of bases in mRNA?
a) TCGAAGCT
b) UGCUAGCT
c) TCGAUCGU
d) UCGAAGCU

11.  Choose the statement which is correct about gene transcription
         a) The transcript is identical to the coding strand and complementary to the template strand 

         b) The transcript is identical to the template strand and complementary to the coding strand 

         c) The transcript is identical to both the coding and template strands 

        d) None of the above

Answers
1. a) Transcription
2. c) RNA polymerase
3. c) DNA to mRNA
4. d) All of these
5. a) Initiates transcription
6. c) RNA polymerase
7. c) Identifies amino acids and transport them to ribosomes
8. d) 5
9. b) Anticodon
10. d) UCGAAGCU
11.  a) The transcript is identical to the coding strand and complementary to the template strand
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