Biotechnology Practice Test on Genetic Engineering (Recombinant DNA Technology)

Genetic engineering is the technology involved in synthesis of artificial genes, repair of genes through fusion, inversion, deletion, shifting of genes, products of recombinant DNA and manipulating them for improvement in human beings, plants, animals and microbes. recombinant DNA technology is employed for combining DNA from two different organisms to produce recombinant DNA.

Biotechnology Practice Test: Recombinant DNA technology
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1. Who discovered recombinant DNA (rDNA) technology?
Har Gobind Khorana
Sutton and Boveri
Stanley Cohen and Herbert Boyer
2. Match the names of the scientists with their contributions and choose the correct answer
 A. Walter Sutton 1) Discovered Penicillin
 B. Stanley Cohen 2) Discovered double helical structure of DNA 
C. Alexander Flemming 3) Discovered the chromosomal basis of heredity

 D. James.D.Watson 4) Discovered r-DNA 
A-2, B-1, C-4, D-3
A-3, B-4, C-1, D-2
A-1, B-3, C-2, D-4
A-4, B-3, C-2, D-1

3. Which of the following is a plasmid?
Sal I
Bam H1
Eco R1

4. Restriction enzymes are
DNA unwinding enzymes
DNA joining enzymes
DNA cleaving enzymes
None of these

5. The enzyme used to join the DNA fragments
DNA ligase
DNA polymerase
Reverse transcriptase

6. Which of the following is used as vector in gene therapy for SCID

7. Ti plasmid used in genetic engineering is obtained from
Bacillus thuringiensis
Agrobacterium rhizogenes
Agrobacterium tumefaciens
Bacillus subtilis

8. The vector for T-DNA is
Thermus aquaticus
Bacillus tumefaciens
Agrobacterium tumefaciens
Escherichia coli

9. cry II Ab and cry I Ab produce toxins that control
Corn borer and tobacco budworms respectively
Nematodes and tobacco budworms respectively
Cotton bollworms and corn borer respectively
Corn borer and cotton bollworms respectively

10. In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (i-iv) in this regard and select the correct option about which one or more of these can be used as a vector/ vectors

 i) Bacterium ii) Plasmid iii) Plasmodium iv) Bacteriophages 
i, ii and iv
ii and iv 
i and iv
i only

Score =
Correct answers:
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