SET Biotechnology Questions (61-80)

61. In C4 plants, Rubisco is localized in

A) Xylem 

B) Phloem

C) Mesophyll cells 

D) Bundle sheath cells

62. The major advantage of radioisotope over fluorescence that makes it more suitable for metabolism studies is:

A) The shelf-life of radioactive compounds are more compared to that of flourochromes used for fluorescent tagging

B) Radio labelling does not cause conformation/structural alteration in a molecule while fluorescent tagging does cause these changes

C) Radio labelling requires less instruments

D) None of these

63. Enzymatic digestion of protein begins in:

A) Large intestine 

B) Small intestine

C) Stomach 

D) Mouth

SET Biotechnology Questions (61-80)

64. Level of ketone bodies increases:

A) In diabetes mellitus 

B) During prolonged exercise

C) During fasting 

D) All of these conditions

65. When a peptide was treated with trypsin, the fragments obtained were proline, asp-lys, and ser-asp-trp-gly-arg. Treatment of the original peptide with flouro, 2, 4 dinitrobenzene followed by acid hydrolysis yielded DNP-Asp. What is the sequence of the original peptide?

A) Pro-ser-asp-trp-gly-arg-asp-lys

B) Asp-lys-pro-ser-asp-trp-gly-arg

C) Asp-lys-ser-asp-trp-gly-arg-pro

D) Ser-asp-trp-gly-arg-asp-lys-pro

66. Proteases are extensively used in:

A) Fruit juice clarification 

B) Detergent industry

C) Pharmaceutical industry 

D) Medical diagnosis

67. Indirect continuous method of industrial sterilization involves:

A) Autoclaving

B) Alcohol treatment

C) Perfect heat exchanging plates

D) Ozone treatment

68. Polarographic electrodes are used in bioreactors to detect:

A) Dissolved oxygen concentration of the medium

B) Conductivity of the medium

C) pH of the medium

D) Dielectric constant of the medium

69. ------ is used as an ingredient in Penicillin production medium.

A) Biotin 

B) Lactamase

C) Phenyl acetic acid 

D) Vitamin B12

70. Terminator gene technology make use of:

A) Cre- lox recombination

B) Flp- frt recombination

C) Lambda site-specific recombination

D) Gateway cloning

71. Triploid plants:

Statement 1. can be produced by culturing endosperms

Statement 2. Triploid plants are seedless

A) 1 is correct and 2 is wrong

 B) 1 is wrong and 2 is correct

C) Both 1 and 2 are correct 

D) Both 1 and 2 are wrong

72. Alpha complementation is:

1. The principle of Blue-white colony selection

2. Complementing a host mutant β- galactosidase

A) 1 & 2 are correct, and 2 is the correct explanation of 1

B) 1 & 2 are correct, but 2 is not the correct explanation of 1

C) 1 is correct, and 2 is wrong

D) 2 is correct, and 1 is wrong8

73. Triparental mating is associated with:

A) Both co integrate and Binary vector system of Agrobacterium tumefaciens

B) Only Binary vector system of Agrobacterium tumefaciens

C) Only co integrate system of Agrobacterium tumefaciens

D) Bac to Bac cloning

74. Topocloning technology completes ligation in:

A) 12 hours 

B) 1 hour 

C) 10 minutes

 D) Half an hour

75. E coli RNA polymerase core enzyme is:

A) αβ2β' 

B) σα2ββ' 

C) σαβ2β' 

D) α2ββ'𝜔

76. Coupled transcription and translation is a feature of :

A) Bacterial protein synthesis

B) Algal Protein synthesis

C) Angiosperm protein synthesis

D) Fungal protein synthesis

77. A twelve-base single-stranded oligonucleotide primer has 3 adenines, 4 guanines, and 3 cytosines. Then calculate the annealing temperature of this primer:

A) 380 C 

B) 480 C 

C) 450 C 

D) 500 C

78. Golden rice contains:

A) Two genes from Narcissus and one gene from Erwinia

B) One gene from Narcissus and two genes from Erwinia

C) Three genes from Narcissus and one gene from Erwinia

D) Three genes from Erwiniaand one gene from Narcissus

79. Taq DNA polymerase is an error-prone polymerase because:

Statement 1. It lacks 3' to 5' exonuclease activity

Statement 2. It lacks proofreading

A) Both 1 and 2 are correct, but they designate two properties

B) Both 1 and 2 are correct, and 1 is the correct explanation of 2

C) Both1 and 2 are correct, but 1 is not the correct explanation of 2

D) 1 is correct, and 2 is wrong

80. Match the following:

a. deamination of adenine 1. Acridine orange

b. deamination of cytosine 2. A-G transition

c. DNA intercalating agent 3. G-A transition

d. DNA cross linking agent 4. Nitrogen mustard

A) a-4, b-3, c-2, d-1 

B) a-3, b-2, c-4, d-1

C) a-2, b-3, c-4, d-1 

D) a-2, b-3, c-1, d-4

Learn more: Biotechnology Topic wise MCQ

Answers

61. D) Bundle sheath cells

62. B) Radio labelling does not cause conformation/structural alteration in a molecule while fluorescent tagging does cause these changes

63.C) Stomach 

64. D) All of these conditions

65. C) Asp-lys-ser-asp-trp-gly-arg-pro

66. B) Detergent industry

67.C) Perfect heat exchanging plates

68. A) Dissolved oxygen concentration of the medium

69.C) Phenyl acetic acid 

70. A) Cre- lox recombination

71. C) Both 1 and 2 are correct

72. A) 1 & 2 are correct, and 2 is the correct explanation of 1

73. A) Both co integrate and Binary vector system of Agrobacterium tumefaciens

74. C) 10 minutes

75. D) α2ββ'𝜔

76. A) Bacterial protein synthesis

77. A) 380 C 

78.A) Two genes from Narcissus and one gene from Erwinia

79. B) Both 1 and 2 are correct, and 1 is the correct explanation of 2

80.D) a-2, b-3, c-1, d-4

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