61. In C4 plants, Rubisco is localized in
A) Xylem
B) Phloem
C) Mesophyll cells
D) Bundle sheath cells
62. The major advantage of radioisotope over fluorescence that makes it more suitable for metabolism studies is:
A) The shelf-life of radioactive compounds are more compared to that of flourochromes used for fluorescent tagging
B) Radio labelling does not cause conformation/structural alteration in a molecule while fluorescent tagging does cause these changes
C) Radio labelling requires less instruments
D) None of these
63. Enzymatic
digestion of protein begins in:
A) Large intestine
B) Small intestine
C) Stomach
D) Mouth
64. Level of ketone
bodies increases:
A) In diabetes mellitus
B) During prolonged exercise
C) During fasting
D) All of these conditions
65. When a peptide was treated with trypsin, the fragments obtained were proline, asp-lys, and ser-asp-trp-gly-arg. Treatment of the original peptide with flouro, 2, 4 dinitrobenzene followed by acid hydrolysis yielded DNP-Asp. What is the sequence of the original peptide?
A) Pro-ser-asp-trp-gly-arg-asp-lys
B) Asp-lys-pro-ser-asp-trp-gly-arg
C) Asp-lys-ser-asp-trp-gly-arg-pro
D) Ser-asp-trp-gly-arg-asp-lys-pro
66. Proteases are
extensively used in:
A) Fruit juice clarification
B) Detergent industry
C) Pharmaceutical industry
D) Medical diagnosis
67. Indirect
continuous method of industrial sterilization involves:
A) Autoclaving
B) Alcohol treatment
C) Perfect heat exchanging plates
D) Ozone treatment
68. Polarographic
electrodes are used in bioreactors to detect:
A) Dissolved oxygen concentration of the medium
B) Conductivity of the medium
C) pH of the medium
D) Dielectric constant of the medium
69. ------ is used as
an ingredient in Penicillin production medium.
A) Biotin
B) Lactamase
C) Phenyl acetic acid
D) Vitamin B12
70. Terminator gene
technology make use of:
A) Cre- lox recombination
B) Flp- frt recombination
C) Lambda site-specific recombination
D) Gateway cloning
71. Triploid plants:
Statement 1. can be produced by culturing endosperms
Statement 2. Triploid plants are seedless
A) 1 is correct and 2 is wrong
B) 1 is wrong and 2 is correct
C) Both 1 and 2 are correct
D) Both 1 and 2 are wrong
72. Alpha
complementation is:
1. The principle of Blue-white colony selection
2. Complementing a host mutant β- galactosidase
A) 1 & 2 are correct, and 2 is the correct explanation of 1
B) 1 & 2 are correct, but 2 is not the correct explanation of 1
C) 1 is correct, and 2 is wrong
D) 2 is correct, and 1 is wrong8
73. Triparental
mating is associated with:
A) Both co integrate and Binary vector system of Agrobacterium tumefaciens
B) Only Binary vector system of Agrobacterium tumefaciens
C) Only co integrate system of Agrobacterium tumefaciens
D) Bac to Bac cloning
74. Topocloning
technology completes ligation in:
A) 12 hours
B) 1 hour
C) 10 minutes
D) Half an hour
75. E coli RNA
polymerase core enzyme is:
A) αβ2β'
B) σα2ββ'
C) σαβ2β'
D) α2ββ'𝜔
76. Coupled
transcription and translation is a feature of :
A) Bacterial protein synthesis
B) Algal Protein synthesis
C) Angiosperm protein synthesis
D) Fungal protein synthesis
77. A twelve-base single-stranded oligonucleotide primer has 3 adenines, 4 guanines, and 3 cytosines. Then calculate the annealing temperature of this primer:
A) 380 C
B) 480 C
C) 450 C
D) 500 C
78. Golden rice
contains:
A) Two genes from Narcissus and one gene from Erwinia
B) One gene from Narcissus and two genes from Erwinia
C) Three genes from Narcissus and one gene from Erwinia
D) Three genes from Erwiniaand one gene from Narcissus
79. Taq DNA
polymerase is an error-prone polymerase because:
Statement 1. It lacks 3' to 5' exonuclease activity
Statement 2. It lacks proofreading
A) Both 1 and 2 are correct, but they designate two properties
B) Both 1 and 2 are correct, and 1 is the correct explanation of 2
C) Both1 and 2 are correct, but 1 is not the correct explanation of 2
D) 1 is correct, and 2 is wrong
80. Match the
following:
a. deamination of adenine 1. Acridine orange
b. deamination of cytosine 2. A-G transition
c. DNA intercalating agent 3. G-A transition
d. DNA cross linking agent 4. Nitrogen mustard
A) a-4, b-3, c-2, d-1
B) a-3, b-2, c-4, d-1
C) a-2, b-3, c-4, d-1
D) a-2, b-3, c-1, d-4
Learn more: Biotechnology Topic wise MCQ
Answers
61. D) Bundle sheath cells
62. B) Radio labelling does not cause conformation/structural alteration in a molecule while fluorescent tagging does cause these changes
63.C) Stomach
64. D) All of these conditions
65. C) Asp-lys-ser-asp-trp-gly-arg-pro
66. B) Detergent industry
67.C) Perfect heat exchanging plates
68. A) Dissolved oxygen concentration of the medium
69.C) Phenyl acetic acid
70. A) Cre- lox recombination
71. C) Both 1 and 2 are correct
72. A) 1 & 2 are correct, and 2 is the correct explanation of 1
73. A) Both co integrate and Binary vector system of Agrobacterium tumefaciens
74. C) 10 minutes
75. D) α2ββ'𝜔
76. A) Bacterial protein synthesis
77. A) 380 C
78.A) Two genes from Narcissus and one gene from Erwinia
79. B) Both 1 and 2 are correct, and 1 is the correct explanation of 2
80.D) a-2, b-3, c-1, d-4
