11. Amino acid composition of protein is determined by
a) Ion exchange Chromatography
b) AAS
c) GIC
d) Electrophoresis
12. Using chromatogram as detector in Chromatography, a graph is obtained between --- and time.
a) quantity
b) density
c) concentration
d) specific gravity
13. Which of the following is the application of ion exchange chromatography?
a) The softening of hardwater
b) The demineralization of water
c) both a and b
d) specific gravity
14. Select the gas which is used as mobile phase in gas chromatography
a) Oxygen
b) Nitrogen
c) Hydrogen
d) Chlorine
15. Which type of chromatographic technique is employed for the purification of large biomolecules of nucleic acids and preoteins?
a) HPLC
b) TLC
c) FPLC
d) GIC
16. Which chromatography method involves a technique in which the staionery phase is held in a narrow tube and the mobile phase is forced through it under pressure?
a) Gas
b) TLC
c) Column Chromatography
d) HPLC
17. Which of the following adsorbent material used in thin layer chromatography?
a) Cellulose
b) Alumina
c) Silica gel
d) All of these
18. The use of insulin hormone to purify its receptor is an example of
a) Ion exchange Chromatography
b) Gel filtration Chromatography
c) Affinity Chromatography
d) Ligand Mediated Chromatography
19. Retardation factor Rf is related to
a) Ion exchange Chromatography
b) Partition Chromatography
c) Thin layer Chromatography
d) HPLC
20. In reverse- phase Partition Chromatography :
a) the stationary phase is nonpolar and the mobile phase is polar
b)
c) both stationary phase and mobile phase are polar
d) both stationary phase and mobile phase are nonpolar
21. Choose the stationary phase in Ion Exchange chromatography from the below given list
a) Silica
b) Alumina
c) Polymer resin
d) StarchIn
22.HPLC, peak area refers to the:
a) Concentration of the compound
b) Absorption maxima of the compound
c) Retention time of the compound
d) None of these
Learn more: MCQ on Biochemistry
Answers:
11.a) Ion exchange Chromatography
12.c) concentration
13. c) both a and b
14. b) Nitrogen
15. c) FPLC
16. c) Column Chromatography
17. d) All of these
18. c) Affinity Chromatography
19. c) Thin layer Chromatography
20.a) the stationary phase is nonpolar and the mobile phase is polar
21. c) Polymer resin
22. a) Concentration of the compound
