1. Which staining method is used to
identify DNA in specimens?
A) Volutin staining
B) Feulgen staining
C) Negative staining
D) Both A and C
2. Mycobacterium tuberculosis is an example for
the---
A) Alcohol fast
B) Acid fast
C) Alcohol and acid fast
D) None of the above
3. Feulgen staining of DNA is based on the reaction
of Schiffs reagent with
A) Free aldehyde group of pentose sugar
B) Purines at N-7 position
C) Pyrimidines at N-1 position
D) Phosphate moiety of nucleotide
4. Osmium tetra oxide is used in electron
microscopy as a
A) Fixing agent
B) Mordant
C) Staining agent
D) Precipitator
5. Systematic essential steps are noticed in the
preparation of paraffin sections before staining has begun. Select the correct
sequence for paraffin embedded sections preparation
A) Fixation -Clearing – Dehydration –Infiltration – Embedding
-Sectioning
B) Fixation – Dehydration-Infiltration –Clearing – Embedding -Sectioning
C) Fixation – Dehydration –Clearing –Infiltration – Embedding -Sectioning
D) Fixation – Dehydration –Clearing- Embedding –Infiltration –Sectioning
6. Which among the following is a neutral stain?
A) Janus Green
B) Methyl Green
C) Borax Carmine
D) Leishman's stain
7. Janus green B is used to stain
A) Chloroplast
B) Golgi complex
C) Vacuoles
D) Mitochondria
8. Sudan Black B is often used for
visualization of
A) Protein
B) Carbohydrates
C) Aminoacids
D) Lipids
9. Which of the following is not a natural
dye?
A) Brazilin
B) Hematoxylin
C) Colchineal
D) Aniline blue
10. ------is an azodye
A) Safranin
B) Haematoxylin
C) Aniline blue
D) Orange G
Answer:
1. B) Feulgen staining
2. C) Alcohol and acid fast
3. A) Free aldehyde group of pentose sugar
4. C) Staining agent
5. C) Fixation – Dehydration –Clearing –Infiltration – Embedding
-Sectioning
6. D) Leishman's stain
7. D) Mitochondria
8. D) Lipids
9. D) Aniline blue
10. C) Aniline blue
