Microbiology MCQ | Microbiologist (Bacteriologist) Exam Questions

1. Aerosol transmission occurs in :

(A) Mycobacterium tuberculosis 

(B) Staphylococcus

(C) Streptococcus 

D) E.coli
2. In a native PAGE, proteins are separated on the basis of :

(A) Net positive charges size 

(B) Net negative charge

(C) Net positive charge 

(D) Net charge and size

3. Light source used in Uv-Vis spectroscopy :

(A) LASER (B) Xenon lamp (C) Sodium vapour lamp (D) Tungsten lamp

4. Mode of action of β-Lactamases in rendering penicillin resistance is by :

(A) Preventing access to the target of the antibiotic

(B) Degrading the antibiotic

(C) Altering the antibiotic

(D) Rapidly extruding the antibiotic

5. One cell takes DNA from the other and inserts it into its chromosome despite the fact that

the two cells are completely unrelated. The procedure is :

(A) Horizontal gene transfer

(B) Transposition

(C) Crossing over of DNA from the two cells

(D) Vertical gene transfer

6. Plasmids are :

(A) Transposons 

(B) Chromosome

(C) Accessory genetic information

 (D) RNA found in bacterial cells

7. The study of genomes recovered from natural samples without first isolating members of the

microbial community and growing them in pure cultures :

(A) Phenomics 

(B) Proteomics

(C) Metabolomics 

(D) Metagenomics

8. The process used in the laboratory to produce millions of copies of DNA is :

(A) In situ polymerization 

(B) Fluctuation test

(C) Polymerase Chain Reaction (PCR) 

(D) Reverse transcriptase

9. Transposons are :

(A) Jumping genes

 (B) Inverted repeat

(C) Insertion sequences 

(D) All of the above


10. Which of the following is not a type of centrifugation?

(A) Disk stack separator 

(B) Microfiltration

(C) Tubular centrifuge

 (D) Hydro cyclone

11. DNA fingerprinting consists of identifying individuals or organisms by their unique :

(A) A series of clones containing the entire genome of a microbe

(B) Recombinant microbial cells

(C) Restriction enzyme fragments of DNA molecules

(D) Single-stranded DNA localized on a substrate

12. Which statement is not true regarding difference between prokaryotes and eukaryotes?

(A) Chromosomes in prokaryotes are circular while linear in eukaryotes

(B) Polyribosomes are present in prokaryotes but not by eukaryotes

(C) Cytoplasm is the site of transcription and translation occur in prokaryotes but not in eukaryotes

(D) Prokaryotes have one copy of each gene while eukaryotes have two copies of each gene

13. Homofermentative lactic acid bacteria lacks the enzyme :

(A) Aldolase

(B) Phosphoketolase

(C) Hexose isomerase 

(D) All of these

14. Phenylacetic acid is added as a precursor to maximize the production of antibiotic :

(A) Penicillin G (B) Streptomycin (C) Both (D) None

15. The amino acids lysine and –––––––––– are used in the food industry as nutritional

supplements in bread products and as flavor enhancing compounds such as monosodium

glutamate.

(A) Glutamic acid (B) Methionine (C) Valine (D) Phenyl alanine

16. Citric acid is produced by the microorganism :

(A) Rhizopus nigricans (B) Aspergillus niger (C) Acetobacter (D) Lactobacillus

17. Antibiotics and mycotoxins falls under which category of microbial products :

(A) Primary metabolites (B) Secondary metabolites (C) Both (D) None

18. In continuous culture techniques using chemostat, cell output is improved by maintain

microorganisms in –––––––––– phase.

(A) Lag (B) Logarithmic (C) Stationary (D) Decline

19. α -amylase is industrially produced from the microorganism :

(A) Bacillus subtilis 

(B) Streptomyces olivaceus

(C) Acetobacter xylinum

 (D) Aspergillus flavus

20. An industrial strain or microorganism should ideally exhibit :

(A) Genetic stability 

(B) Safety, non-pathogenicity

(C) Efficient production

 (D) All of these


Answers:

1-A

2-D

3-B

4-B

5-A

6-C

7-D

8-C

9-D

10-B

11-C

12-B

13-B

14-D

15-A

16-B

17-B

18-B

19-A

20-D

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