SET Biotechnology Practice Test 4

31. The primary purpose of using a hot start PCR is to:

A) Increase specificity B) Reduce non specific binding C) Prevent primer dimer formation D) Enhance Taq activity

32. After cleaving the sequence, the nature of the ends created by the type II endonuclease is :

A) The ends created are always single stranded B) The ends created are always double stranded C) Either the ends are single stranded or they are double stranded D) One end is single stranded and one end is double stranded

33. The purpose of guide RNA in CRISPR-Cas9 is to:

A) Cleave target DNA B) Repair double strand DNA breaks C) Target specific DNA sequences D) Activate gene expression

34. Choose the most popular enzyme used in RFLP analysis due to its ability to recognise methylation sensitive sites:

A) HindIII B) MseI C) HpaII D) EcoRI

35. Identify the correct codes of the various steps for construction of libraries:

1. Fragmentation of DNA
2. Isolation of genomic DNA
3. Amplification
4. Ligation and introduction to the host
5. Vector preparation

A) 1, 2, 3, 4, 5 B) 2, 1, 5, 4, 3 C) 1, 2, 5, 3, 4 D) 2, 5, 1, 4, 3

36. Choose the incorrect statement for oligo-dT cellulose.

A) It is used for separation of polyadenylated mRNA from other species of RNA B) oligo-dT are covalently attached to the solid support via OH bonds C) A solution containing RNA is passed through the column D) Poly A tail attaches to the oligo-dT by ionic bonds

37. Identify the correct statements on nitrogenase enzyme used for nitrogen fixation:

1. Nitrogenase mediated nitrogen fixation is an energetically expensive process.
2. The gene encoding Nitrogenase is under a constitutive promoter
3. Nitrogenase is highly sensitive to oxygen
4. Endogenous availability of cofactors of nitrogenase is very low.

A) 1 & 2 only B) 1 & 3 only C) 2 & 3 only D) 2 & 4 only

38. Give the names of enzymes required - in plant : somatic cell hybridization to prepare protoplast, in animal cell culture: to prepare cell suspension :

A) Amylase and phospholipase B) Cellulase and trypsin C) Lipase and dipeptidase D) Catalase and galactosidase

39. Identify the Ti plasmid vector, commonly used for binary vector systems:

A) pTiA6 B) pTiT37 C) pGV2260 D) pBIN19

40. If a manufacturing company shows non compliance with CGMP regulations:

A) Any drug manufactured by that company will be considered “adulterated” B) No actions will be taken if the drugs are safe C) The company will be closed instantly after inspection D) It means that there is necessarily a problem with the quality of the drug