Biology Multiple Choice Questions and Answers for Different Competitive Exams

Multiple Choice Questions on Gram Staining

1. Gram staining was developed by
a) French microbiologist Louis Pasteur
b) Dutch lens maker Leeuwenhoek
c) Danish physician Christian Gram
d) Dutch physician Christian Gram

2. Gram staining is an example of
a) Acid fast stain
b) Acid stain
c) Differential stain
d) None of the above

3. Gram staining was developed in
a) 1882
b) 1883
c) 1884
d) 1885

4. The most common stains used in Gram staining is
a) crystal violet and methylene blue
b) crystal violet and safranin
c) crystal violet and carbol fuschin
d) safranin and methylene blue

gram positive and gram negative cell wall layers
Bacterial cell wall
5. Which of the following statements are true regarding Gram positive bacteria
a) cell wall has a thick peptidoglycan layer
b) cell wall lipid content is very low
c) lipopolysaccharide layer is absent
d) all of these

6. Which of the following statements is incorrect regarding Gram negative bacteria
a) cell wall has a thin peptidoglycan layer
b) cell wall lipid content is very low
c) lipopolysaccharide layer is present
d) all of these

7. In Gram staining, if some bacteria retain the crystal violet stain after alcohol treatment. Then the bacteria is
a) Gram positive
b) Gram negative
c) procedure is incomplete to answer this question
d) none of these

8. Counter stain used in Gram staining is
a) Safranin
b) Crystal violet
c) carbol fuschin
d)acetocarmine

9. In Gram staining , the alcohol acts on
a) Teichoic acids
b) Periplasm
c) Membrane lipids
d) Peptidoglycan
10. Lipopolysaccharide is found in cell wall of
a) Gram positive bacteria
b) Gram negative bacteria
c) Both
d) Fungi

11. The diffrential staining property of  Gram staining is primarily due to
a) difference in lipid content in Gram positive and Gram negative bacteria 
b) difference in protoplasmic contents in Gram positive and Gram negative bacteria
c) difference in teichoic acid content in Gram positive and Gram negative bacteria
d) all of these

12. In Gram staining iodine is used as a
a) fixative
b) mordant
c) solubiulizer
d) stain

13.  After ethanol treatment Gram negative bacteria can be visualised
a) only by counter staining with safranine
b) in violet colour of crystal violet
c) only by addition of iodine solution
d) none of these

14. Which of the following is a common Gram positive bacteria
a) Rhizhobium of root nodules
b) Lactobacillus in curd
c) Eschericia coli
d) none of these 

15. During ethanol treatment in gram staining,
a) crystal violet stain is dissolved in alcohol in  Garm negative bacteria due to high lipid content in the outer layer
b) crystal violet stain is retained in Garm positive bacteria due to less lipid content and thick peptidoglycan wall
c) both a and b
d) none of these

1. c) Danish physician Christian Gram
2. c) Differential stain
3. b) 1883
4. b) crystal violet and safranin
5. d) all of these
6. b) cell wall lipid content is very low
7. a) Gram positive
8. a) Safranin
9. c) Membrane lipids
10. b) Gram negative bacteria
11. a) difference in lipid content in Gram positive and Gram negative bacteria
12. b) mordant
13. a) only by counter staining with safranine
14. b) Lactobacillus in curd
15. c) both a and b
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