1. Any DNA molecule that has the ability to replicate in an appropriate
host cell, to which the desired gene are integrated for cloning, is called as
a) Plasmid
b) linker
c) vector
d) adapter
2. Which of the following statement is true
a) a vector should have an origin of
replication
b) a vector should have selectable
markers
c) a vector should have unique
restriction sites
d) all of these
3. Expression vectors differ from a cloning vector in
having
a) an origin of replication
b) suitable marker genes
c) unique restriction sites
d) control elements
4. Extra chromosomal, double stranded, circular DNA
molecule present in bacteria which is widely used as vector is called
a) phagemid
b) cosmid
c) plasmid
d) bacterial vectors
5. The first engineered plasmid vector is
a) pBR 322
b) pUC vectors
c) pSC101
d) pUC 19
6. The most popular and widely used engineered plasmid
vector is
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pBR 322 |
a) pBR 322
b) pUC vectors
c) pSC101
d) pUC 19
7. In pBR 322, pBR stands for
a) plasmid bacterial recombination
b) plasmid bacterial replication
c) plasmid Boliver and Rodriguez
d) plasmid Baltimore and Rodriguez
8. Phage M13 vectors are widely used for
a) obtaining single stranded copies of cloned
DNA suitable for DNA sequencing
b) obtaining double stranded copies of
cloned DNA suitable for DNA sequencing
c) obtaining fragments of cloned DNA
suitable for DNA sequencing
d) obtaining double stranded copies of cloned
DNA suitable for electrophoresis
9. Cosmid is a plasmid with
a) a minimum of 250 bp of lambda DNA that
includes cos site
b) a minimum of 250 bp of M13 DNA that
includes cos site
c) a minimum of 100 bp of lambda DNA
that includes cos site
d) a minimum of 250 bp of T4 phage DNA that
includes cos site
10. Vectors designed to replicate in cells of two
different species are called
a) phasmids
b) transfer vectors
c) shuttle vectors
d) phagemids
11. Autonomously replicating sequences (ARS) is a
characteristic feature of
a) plasmid vectors
b) phage vectors
c)
E.coli vectors
d) yeast vectors
12. Which of the following are vectors for animals
a) SV 40 vectors and Bovine papillomavirus
vectors
b) CMV vectors and Gemini vectors
c) lambda phage and M13 phage vectors
d) all of the above
13. The vectors commonly used for sequencing human genome
a) Yeast artificial chromosome (YAC)
b) plasmid
c) CMV vectors
d) M13 vectors
14. Ti plasmids that is used
as a plant vector is obtained from
a) Agrobacterium
tumefaciens
b) Agrobacterium
rhizhogenes
c) Agrobacterium
radiobactor
d) Thermus aquaticus
15. The most common vectors for plants are
a) SV 40 vectors and Bovine papillomavirus
vectors
b) Cauliflower mosaic virus (CMV
vectors) and Gemini vectors
c) lambda phage and M13 phage vectors
d) T4 phage vectors
Answers:
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1-c
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2-d
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3-d
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4-c
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5-c
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6-a
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7-c
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8-a
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9-a
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10-c
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11-d
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12-a
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13-a
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14-a
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15-b
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Tags:
biotechnology multiple choice
Biotechnology Practice Test
mcq on cloning vectors
mcq on gene cloning vectors
mcq on vector