1. The
virus mediated gene transfer using genetically modified bacteriophages is
called
a)
transfection
b) transduction
c) transformation
d) conjugation
2. The ability of cells to take up DNA fragments
from surrounding is called
a)
transfection
b) transduction
c) transformation
d) conjugation
3. Which
of the following bacterium is considered as ‘natural genetic engineer’
a)
Agrobacterium tumefaciens
b) Agrobacterium
radiobactor
c) Psueudomonas
putida
d) Thermus
aquaticus
4. The
removal or replacement of tumor causing genes from Ti plasmid is termed as
a)
gene replacement
b) disarming
c) insertional inactivation
d)
gene displacement
5. Agrobacterium tumefaciens is
a
 |
| Crown gall disease |
a)
Gram negative soil bacterium causing crown gall disease in dicots
b) Gram negative soil bacterium causing crown gall
disease in monocots
c) Gram positive soil bacterium causing crown gall
disease in dicots
d)
Gram positive soil bacterium causing crown gall disease in dicots
6. Which
of the following statements are true for agrobacterium mediated gene transfer
a)
Vir genes are essential for gene transfer
b) T-DNA borders are essential for gene transfer
c) both a and b
d)
none of these
7. Ti
plasmid vectors include
a)
binary vectors and cointegrate vectors
b) cointegrate vectors and multiple vectors
c) multiple vectors and binary vectors
d)
Ti plasmid based vector
8. Which
of the following chemical enhances vir
gene expression
a)
cyanidin
b) glutennin
c) acetosyringone
d)dextran
9. Chemicals
used for gene transfer methods include
a)
poly ethylene glycol
b) CaCl2
c) dextran
d)
all of the above
10. Introduction
of DNA into cells by exposing to high voltage electric pulse is
a)
electrofusion
b) elctrofision
c) elctrolysis
d)
electroporation
11. The
transformation method that uses tungsten or gold particle coated with DNA
accelerated at high velocity is called
 |
a)
acceleration method
b) high velocity method
c) particle gun delivery method
d)
DNA particle delivery method
12. The
method widely used for transforming invitro
animal cell cultures that uses lipid vescicles or liposomes
a)
lipotransformation
b) liposome mediated transformation
c) lipofection
d)
lipid mediated DNA transfer
13.
DNA solution injected directly into the cell using micromanipulators is called
a)
macroinjection
b) micromanipulator mediated DNA delivery
c) microfection
d)
microinjection
14.
Fibre mediated DNA delivery uses
a)
silicon carbide fibres that will create pores in the membrane
b) aluminium carbide fibres that will create pores in the
membrane
c) boron carbide fibres that will create pores in the
membrane
d)
lead carbide fibres that will create pores in the membrane
15. The
injection of DNA into developing inflorescence using a hypodermic syringe is
called
a)
macroinjection
b) micromanipulator mediated DNA delivery
c) microfection
d) microinjection
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Answers:
1-b
|
2-c
|
3-a
|
4-b
|
5-a
|
6-c
|
7-a
|
8-c
|
9-d
|
10-d
|
11-c
|
12-c
|
13-d
|
14-a
|
15-a
|