Multiple Choice Questions on DNA Replication

1. Which of the following enzymes are used to join bits of DNA?

a) DNA ligase
b) DNA polymerase
c) primase
d) Endonuclease

2. Semi conservative replication of DNA was first demonstrated in

a) Escherichia coli
b) Streptococcus pneumonae
c) Salmonella typhimuriam
d) Drosophila melanogaster

3. Mode of DNA replication in E.coli is

a) Conservative and unidirectional
b) Semiconservative and unidirectional
c) Conservative and bidirectional
d) Semiconservative and bidirectional

4. When DNA replication starts

a) The phosphodiester bonds between the adjacent nucleotides break
b) The bonds between the nitrogen base and deoxyribose sugar break
c) The leading strand produces Okazaki fragments
d) The hydrogen bonds between the nucleotides of two strand break

5. During the replication of DNA, the synthesis of DNA on lagging strand takes place in segments, these segments are called

a) Satellite segments
b) Double helix segments
c) Kornbeg segments
d) Okazaki segments

6. The elongation of the leading strand during DNA synthesis

a) Progresses away from the replication fork
b) Occur in 3’-5’ direction
c) Produces Okazaki fragment
d) Depend on the action of DNA polymerase

7. Eukaryotes differ from prokaryote in mechanism of DNA replication due to:

a) Different enzyme for synthesis of lagging and leading strand
b) Use of DNA primer rather than RNA primer
c) Unidirectional rather than bidirectional replication
d) Discontinuous rather than semidiscontinuous replication

8. True replication of DNA is possible due to

a) Hydrogen bonding
b) Phosphate backbone
c) Complementary base pairing rule
d) None of the above

9. Unwinding of DNA is done by

a) Helicase
b) ligase
c) Hexonuclease
d) Topoisomerase

10. DNA synthesis can be specifically measured by estimating the incorporation of radio labelled

a) uracil
b) thymine
c) Adenine
d) Deoxyribose sugar

Free online MCQ Test on: Transcription -> Translation -> Protein Synthesis
Answers

1. DNA ligase
2. a) Escherichia coli
3. d) Semiconservative and bidirectional
4. d) The hydrogen bonds between the nucleotides of two strand break
5. d) Okazaki segments
6. d) Depend on the action of DNA polymerase
7. d) Discontinuous rather than semidiscontinuous replication
8. c) Complementary base pairing rule
9. a) Helicase 

10. b) thymine

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