Multiple Choice Questions on Genetic Engineering and Recombinant DNA technology

1. DNA sequencing is done by
DNA sequencing
a) Maxam Gilbert method
b) Sanger dideoxy method
c) Both a and b
d) Watson and Crick

2. A vector should have which of the following properties
i) MCS                   ii) Small size  iii) Multiple ori  iv)low replication speed
a) i, ii, iii               
b) ii, iii, iv
c) i, ii, iv
d) i, iii, iv

3. DNA staining is done by
a) Cystal violet
b) Giemsa staining
c) Methylene blue
d) Feulgen staining

4. Which of the following is not a restriction endonuclease?
a) Eco RI
b) DNA ligase
c) Hind III
d) Bam H1

5. Use of alkaline phosphatase is to
a) remove terminal phosphates from 3’end
b) remove terminal phosphates from 5’end
c) remove terminal phosphate from both end
d) All of these

6. The uptake of plasmid DNA into bacterial cell is facilitated in the presence of
a) calcium chloride
b) magnesium chloride
c) potassium chloride
d) all of these

7. Taq polymerase is used in PCR because of its
a) low thermal stability
b) high fidelity
c) high speed
d) high thermal stability

8. Introduction of recombinant DNA into bacterial cell by using current is called
a) transformation
b) electroporation
c) transformation
d) tranduction

9. Nick translation is done by
a) DNA polymerase I
b) DNA polymerase II
c) DNA ligase
d) Kinase

10. Telomeric sequences are found in
a) HAC
b) BAC
c) YAC
d) PAC
1. c) Both a and b
2. c) i, ii, iv
3. d) Feulgen staining
4. b) DNA ligase
5. d) all of these
6. d) all of these
7. d) high thermal stability
8. b) electroporation
9. a) DNA polymerase I
10. c) YAC


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  1. This is regarding Bacterial transformation(Question no. 6)
    All the mentioned components are used in the buffers for transformation( Reference Shambrook and Russel)
    KCl is used in making Transformation Buffer(TFB) whereas a solution of CaCl2 and MgCl2 is used or making competent cells.

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