Multiple Choice Questions on PCR Types || Molecular Biology Techniques MCQ

1. Which of the following PCR technique is best suited for detection of point mutation in DNA?

a) RT PCR

b) PCR

c) Allele specific PCR

d) Anchored PCR

2. The PCR technique used to preferentially amplify only one strand of the target DNA by using unequal primer concentration is   

a) qPCR

b) Asymmetric PCR

c) Anchored PCR

d)  Nested PCR

3. The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is

a) Anchored PCR

b) Colony PCR

c) qPCR

d) pPCR

4. This PCR variant uses degenerate primers to amplify unknown sequences of DNA, related to a known DNA sequence is

a) Degenerate PCR

b) Multiplex PCR

c) Hotstart PCR

d) Inverse PCR

5. If the sequence of only one strand is known for primer synthesis, the best PCR variant that allows amplification of DNA with only one known sequence is

a) Hotstart PCR

b) Inverse PCR

c) Multiplex PCR

d) Nested PCR

6. The most efficient PCR technique that uses primer mix to detect deletions or duplications in a large gene is  

a) Anchored PCR

b) Nested PCR

c) Miniprimer PCR

d) Multiplex PCR

7. The PCR technique designed to minimize amplification of non-specific PCR products by using 2 primer sets is

a) Multiplex PCR

b) Mini primer PCR

c) Nested PCR

d) q PCR

8. The PCR variant designed for the study of RNA expression level is  

a) Reverse transcriptase or RT PCR

b) q PCR

c) Anchored PCR

d)  Traditional PCR

9. The following PCR technique allows measuring the DNA amplification at each cycle of PCR contrary to end point detection in traditional PCR

a) q PCR or Quantitative PCR

b) Reverse transcriptase or RT PCR

c) Asymmetric PCR

d) touchdown PCR

10. The PCR procedure, where Taq polymerase is added after the rest of the PCR components are heated to the DNA melting temperature, so as to avoid non-specific amplification at lower temperatures is

a) Asymmetric PCR

b) Hotstart PCR

c) Multiplex PCR

d) Nested PCR

11. Type of PCR used for the detection and measurement of products generated during each cycle of PCR process:

a) qRT-PCR

b) Assembly PCR

c) Multiplex PCR

d) Nested PCR

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Answers:

1. c) Allele specific PCR

2. b) Asymmetric PCR

3. b) Colony PCR

4 . a) Degenerate PCR

5. b) Inverse PCR

6. d) Multiplex PCR

7. c) Nested PCR

8. a) Reverse transcriptase or RT PCR

9. a) q PCR or Quantitative PCR

10. b) Hotstart PCR

11. a) qRT-PCR