Multiple Choice Questions on Chromatography | Separation Techniques Part 1

1. Separation of different fatty acids by

a) Counter current chromatography

b)affinity chromatography

c)gas chromatography

d)Thin layer chromatography

 

2. Silica gel G is used in

a) Column chromatography

b) Gas chromatography

c)Thin layer chromatography

d)HPLC

3. In-gel permeation chromatography,---is eluted first from the column.

a) Cations

b) Anions

c)Bigger molecules

d)smaller molecules

4. Proteins maybe separated according to size by

a) isoelectric focusing

b) molecular exclusion chromatography

c)ion-exchange chromatography

d)reverse phase chromatography

5. Match the following

P. Mixture of glycine and albumin            1. Gas Chromatography

Q. Mixture of 20 and 60kDa proteins       2. Dialysis

R. Histones from nuclear extract             3. Affinity Chromatography

S. Lectins                                                 4. Size exclusion chromatography

                                                                 5. Thin layer chromatography

                                                                 6.Cation exchange chromatography

a) P-1, Q-4, R-3, S-5

b) P-5, Q-3, R-3,S-1

c) P-2, Q-4, R-6, S-3

d) P-6, Q-5 ,R-2, S-4

6. A globular protein of molecular weight of 50 kDa exists as a mixture of monomers and dimers in solution. The most appropriate technique for the separation of these two forms of the protein is

a) gel filtration Chromatography

b) ion-exchange Chromatography

c) thin layer Chromatography

d) paper Chromatography

 

7. Chromatography that involves the separation of isomers

a) Thin layer Chromatography

b) count current Chromatography

c) Chiral Chromatography

d) paper Chromatography

8. The total volume of material, both solid and liquid in the column is known as

a) Void volume
b) Bed volume

c) Elution volume
d) Retention volume

9. In which chromatography, stationary phase is held in a narrow tube and the mobile phase is forced through under pressure?

a) Thin layer Chromatography

b) Column Chromatography

c) High Pressure Liquid Chromatography

d) Paper Chromatography

10. Isolation of eukaryotic mRNA from a mixture of total cellular RNA is carried out by---technique.

a) Thin layer Chromatography

b) Column Chromatography

c) Affinity  Chromatography

d) Immuno blotting  Chromatography

Learn more: MCQ on Biochemistry

Answers:

1. c)gas chromatography

2. c)Thin layer chromatography

3. c)Bigger molecules

4. b) molecular exclusion chromatography

5. c) P-2, Q-4, R-6, S-3

6. a) gel filtration Chromatography

7. c) Chiral Chromatography

8. B) Bed volume

9. c) High Pressure Liquid Chromatography

10. c) Affinity  Chromatography