MCQ on PCR (Polymerase Chain Reaction)

1. The PCR technique was developed by 

a) Kary Mullis
b) Kohler
c) Milstein
d) Altman

2. PCR is a 

a) DNA degradation technique
b) DNA amplification technique
c) DNA sequencing technique
d) all of these

3. Which of the following statements are true regarding PCR

a) billions of copies of desired DNA can be synthesized from microgram quantities of DNA
b) Automated PCR machines are called thermal cyclers
c) A thermostable DNA polymerase is required
d) all of these

4. Thermus aquaticus is the source of

a) Taq polymerase
b) Vent polymerase
c) both a and b
d) primase enzyme

5. All the following are thermostable polymerases except

a) Taq polymerase
b) Vent polymerase
c) DNA polymerase III
d) pfu polymerase

6. The basic requirements of PCR reaction include

a) DNA segment to be amplified
b) two oligonucleotide primers
c) a heat stable DNA polymerase
d) all of these

7. Pfu and Vent polymerase are more efficient than Taq polymerase because

a) of more efficient polymerase activity
b) of proof reading activity
c) both a and b
d) none of these

8. The first step of PCR is 

a) denaturation
b) annealing
c) primer extension 

d) none of these 

Steps in PCR

9. Denaturation involves

a) heating between 90-98⁰ C
b) heating between 40-60⁰ C
c) heating between 72⁰ C
d) none of these

10. The process of binding of primer to the denatured strand is called

a) denaturation
b) annealing
c) renaturation
d) none of these

11. Which of the following is true regarding PCR

a) denaturation involves heating at 90-98⁰ C
b) annealing involves binding of primer between 40-60⁰ C
c) Primer extension occurs at 72⁰ C
d) all of these

12. Asymmetric PCR is

a) used to generate single stranded copies for DNA sequencing
b) used to generate double stranded copies for DNA sequencing
c) both a and b
d) none of these

13. PCR is used in

a) site specific recombination
b) site directed mutagenesis
c) both a and b
d) site specific translocation

14. Reverse transcriptase PCR uses

a) mRNA as a template to from cDNA
b) RNA as a template to from DNA
c) DNA as a template to from ssDNA
d) all of these

15. PCR is used to

a) amplify gene of interest
b) construct RAPD maps
c) detect the presence of transgene in an organism
d) all of these

Answers

1. a) Kary Mullis
2. d) all of these
3. d) all of these
4. a) Taq polymerase
5. a) Taq polymerase
6. d) all of these
7. b) of proof reading activity
8. a) denaturation
9. a) heating between 90-98⁰ C
10. b) annealing
11. d) all of these
12. a) used to generate single stranded copies for DNA sequencing
13. b) site directed mutagenesis
14. a) mRNA as a template to from cDNA
15. d) all of these