MCQ on Plant Biotechnology and Tissue Culture | Biotechnology MCQs

1. The ability of a single cell to divide and produce all the differentiated cells in an organism is called: 

 A) Somatic embryogenesis 

B) Totipotency 

C) De-differentiation 

D) Somaclonal variation 

2. Which of the following is true regarding Downstream processing

A) The cost of downstream processing is more than the manufacturing cost

B) Rotary drum vacuum filters are commonly used in downstream processing

C) Flocculation and floatation are used for the recovery of microbial biomass 

D) All of the above

 3. Development of brittle, glassy and water-soaked shoot under in vitro conditions is called:

A) Morphological variations

B) Somaclonal variations 

C) Vitrification 

D) Guttation 

4. Production of virus-free germplasm can be accomplished by:

A) Haploid culture

B) Embryo culture 

C) Meristem culture 

D) Somatic embryogenesis

5. Choose the statement/s which are correct about anther culture

1. In pathway I uninucleate pollen divides symmetrically and both the vegetative and generative cells undergo further division.

2. In pathway II uninucleate pollen divides unequally and embryos originate from the generative cell alone.

 3. In pathway III uninucleate pollen divides unequally and the embryo originates from the vegetative cell alone.

4. In pathway IV uninucleate pollen divides unequally and both the vegetative and generative cells undergo further division 

A) 1 & 4 only B) 1, 2, 3 &4 C) 2 & 3 only D) 1, 2 & 4 only 

6. The most commonly used fusogen for protoplast fusion is

A) Pectinase

B) High pH and low Ca 2+

C) Polyethylene glycol 

D) Low pH and high Ca 2+ 

7. Cybrids are: 

A) Plants with the nucleus of one species but cytoplasm from both parental species 

B) Plants with the cytoplasm of one species but nucleus from both parental species

C) Plants with nucleus and cytoplasm from both parental species

 D) None of the above 

8. Border sequences need to be incorporated into the design of plasmid vectors for Agrobacterium-mediated transformation to ensure:

A) Greater promoter efficiency B

B) Oncogene deactivation

C) Efficient replication of the plasmid 

D) Integration of the gene of interest into the host gene 

9. Match Group I with Group II

Group I                  Group II

a. Azadirachtin 1. Digitalis lanata

b. Digoxin        2. Taxus buccata

c. Taxol           3. Dioscorea deltoidea

d. Diosgenin  4. Azadirachta indica

A) a-4, b-3, c-2, d-1

B) a-4, b-2, c-1, d-3

C) a-3, b-4, c-2, d-1 

D) a-4, b-1, c-2, d-3

10. GMPs refers to:

A) Global Monitoring Practices

B) Genetically Modified Plants 

C) Good Manufacturing Practices 

D) Guidance for Manufacturing Pharmaceuticals 

11. Flavr Savr tomatoes were developed by introducing an antisense copy of the gene for:

A) Polyhydroxybutyrate

B) Polygalacturonase 

C) EPSP

D) Lycopene cyclase Question

12. Which among the following is NOT a transfection method?

A) Lipofection

B) Shotgun method

C) Colony hybridization

D ) Shotgun method

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• Topic Wise MCQ on Biotechnology 

Answers:

1. B) Totipotency 

2. D) All of the above

3. C) Vitrification 

4. C) Meristem culture 

5. A) 1 & 4 only

6. C) Polyethylene glycol 

7. A) Plants with the nucleus of one species but cytoplasm from both parental species 

8. D) Integration of the gene of interest into the host gene 

9. D) a-4, b-1, c-2, d-3

10. C) Good Manufacturing Practices 

11.  B) Polygalacturonase 

12. C) Colony hybridization