Multiple Choice Questions on Lysosomes

MCQ on lysosome (Gr., lyso=digestive +soma=body)
1. Which of the following organelle is called as the “perinuclear dense bodies”?
a) lysosome
b) nucleolus
c) peroxisome
d) all of these

2. Lysosomes are present in all except
a) muscle cells
b) acinal cells
c) erythrocytes
d) hepatocytes

3. Which is the enzyme used as a marker for the lysosomes
a) Pyruvate dehydrogenase
b) Phospholipase
c) Acid phosphatase
d) Succinate dehydrogenase

4. The pH of lysosome is
a) neutral
b) acidic
c) basic
d) depends on the cell type

5. The staining technique used to locate lysosome is
a) Janus Green staining
b) Bismarck stain
c) Gamori stain
d) Osmium tetroxide

6. Liposomal fractions are isolated by
a) electrophoresis
b) ultra centrifugation
c) both a and b
d) Sucrose density gradient centrifugation

7. The acidic pH of lysosome is due to
a) deposition of waste materials
b) presence of hydrolytic enzymes which has an acidic optimum pH
c) presence of anabolic enzymes which has an acidic optimum pH
d) All of these

8. Which of the following organelle is called as the “suicidal bags of the cell”?

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a) lysosomes
b) lysosomes and Golgi
c) lysosomes and ER
d) lysosomes and mitochondria

9. Which of the following is the function of lysosomes
a) autophagy
b) autolysis
c) digestion
d) all of these


10. During protein targeting, lysosomal proteins are tagged with
a) sialic acid
b) KDEL sequence
c) mannose -6-phosphate
d) glycolipids

11. Which of the following is not a diseases associated with lysosome malfunctioning
a) Tay-Sachs disease
b) I-cell disease
c) Pompe’s disease
d) Marfan syndrome

12. lysosomes are involved in
a) Extracellular digestion
b) intracellular digestion
c) both a and b
d) none of these

13. Lysosomes involved in extracellular digestion are present in
a) cardiac cells
b) sperm
c) zygote
d) All of these

14. The acidic pH in the lysosome is maintained by
a) GTP dependent proton pump in the lumen
b) ATP dependent proton pump on the membrane
c) HCl in lysosome
d) all of these

15. Lysosomal membrane is rich in
a) cardiolipin
b) sialic acid
c) sterols
d) all of these
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Answers
1. a) lysosome
2. c) erythrocytes
3. c) Acid phosphatase
4. b) acidic
5. c) Gamori stain
6. d) Sucrose density gradient centrifugation
7. b) presence of hydrolytic enzymes which has an acidic optimum pH
8. a) lysosomes
9. d) all of these
10. c) mannose -6-phosphate
11. d) Marfan syndrome
12. c) both a and b
13. b) sperm
14. b) ATP dependent proton pump on the membrane
15. b) sialic acid

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