Multiple Choice Questions on Microscopy Basics

1. When the power of ocular lens is 10 X and objective lens is 20 X, the magnification is
a) 30 times
b) 20 times
c) 200 times
d) 2000 times
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2. Which of the following light is suitable for getting maximum resolution?
a) Red
b) Green
c) Blue
d) Orange


3. All the following are components of compound microscope except
a) stage clips
b) fine adjustment
c) electron gun
d) Binocular eye piece

4. Resolving power of an instrument can be increased by
a) using an illumination of longer wavelength and by decreasing the NA
b) using an illumination of longer wave length and by increasing the NA
c) using an illumination of shorter wave length and by increasing the NA
d) using an illumination of shorter wave length and by decreasing the NA

5. Which of the following is best suited to get the surface view of an object?
a) SEM
b) TEM
c) both a and b
d) compound microscope

6. The resolving power of unaided human eye is
a) 1 cm
b) 100um
c) 200 nm
d) 400 nm

7. Which of the following is used to visualize live cells?
a) SEM
b) TEM
c) phase contrast microscope
d) All of these

8. The refractive index of air is
a) 0.50
b) 0.75
c) 1.00
d) 1.25

9. The instrument used to draw clear magnified sketches of objects under microscope is
a) Compound microscope
b) Light microscope
c) camera lucida
d) camera attached stereomicroscope

10. Which of the following is true about dark field microscopy?
a) Adding disc called ‘stop’ to the condenser will make bright field to dark filed
b) The stop disc prevents the entry of light from the central field and object is illuminated with beam of light
c) The light gets reflected from the sides of the specimen and appears bright in dark background
d) all of these

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Answers
1. c) 200 times
2. c) Blue
3. c) electron gun
4. c) using an illumination of shorter wave length and by increasing the NA
5. a) SEM
6. b) 100um
7. c) phase contrast microscope
8. c) 1.00
9. c) camera lucida
10. d) all of these

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