Biology Multiple Choice Questions and Answers for Different Competitive Exams

MCQ on Human Diseases

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MCQ on Bioinformatics

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MCQ on Evolution

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MCQ on Biophysics

1. Set- 1
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MCQ on Biotechnology

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MCQ on Biotechnology - Plant Tissue Culture

Plant Tissue Culture
1. Who is the father of tissue culture?
a) Bonner
b) Haberlandt
c) Laibach
d) Gautheret
2. The production of secondary metabolites require the use of
a) protoplast
b) cell suspension
c) meristem
d) auxillary buds
3. Synthetic seed is produced by encapsulating somatic embryo with
a) sodium chloride
b) sodium alginate
c) sodium acetate
d) sodium nitrate
4. Hormone pair required for a callus to differentiate are
a) auxin and cytokinin
b) auxin and ethylene
c) auxin and absiccic acid
d) cytokinins and gibberllin
5. DMSO (Dimethyl sulfoxide) is used as
a) Gelling agent
b) alkaylating agent
c) Chelating agent
d) Cryoprotectant
6. The most widely used chemical for protoplast fusion, as fusogens, is
a) Manitol
b) Sorbitol
c) Mannol
d) Poly ethylene glycol (PEG)
7. Cybrids are produced by
a) Fusion of two different nuclei from two different species
b) Fusion of two same nuclei from same species
c) Nucleus of one species but cytoplasm from both the parent species
d) None of the above
8. Callus is
a) Tissue that forms embryo
b) An insoluble carbohydrate
c) Tissue that grows to form embryoid
d) Un organised actively dividing mass of cells maintained in cultured
9. Part of plant used for culturing is called
a) Scion
b) Explant
c) Stock
d) Callus
10. Growth hormone producing apical dominance is
a) Auxin
b) Gibberellin
c) Ethylene
d) Cytokinin
11. A medium which is composed of chemically defined compound is called
a) Natural media
b) Synthetic media
c) Artificial media
d) None of these
12. To obtain haploid plant, we culture
a) Entire anther
b) Nucleus
c) Embryo
d) Apical bud
13. Somaclonal variations are the ones
a) Caused by mutagens
b) Produce during tissue culture
c) Caused by gamma rays
d) Induced during sexual embryogeny
14. Which of the following plant cell will show totipotency?
a) Xylem vessels
b) Sieve tube
c) Meristem
d) Cork cells
15. Which vector is mostly used in crop improvement?
a) Plasmid
b) Cosmid
c) Phasmid
d) Agrobacterium

  1. b) Haberlandt
  2. b) cell suspension
  3. b) sodium alginate
  4. a) auxin and cytokinin
  5. d) Cryoprotectant
  6. d) Poly ethylene glycol (PEG)
  7. c) Nucleus of one species but cytoplasm from both the parent species
  8. d) Un organised actively dividing mass of cells maintained in cultured
  9. b) Explant
  10. a) Auxin
  11. b) Synthetic media
  12. a) Entire anther
  13. b) Produce during tissue culture
  14. c) Meristem
  15. d) Agrobacterium
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Multiple Choice Questions on Plant Physiology - Respiration

Respiration in plants
CH2O + O2 → CO2 + H2O + energy
1. The process of respiration in green plants occurs
a) only when stomata are open
b) only when photosynthesis ceases
c) only when photosynthesis is in progress
d) At all times

2. Respiratory enzymes are located in
a) mitochondrial matrix
b) cristae
c) perimitochondrial space
d) outer membrane

3. The site of glycolysis in a cell is
a) chloroplast
b) nucleus
c)  cytoplasm
d) mitochondria

4. Respiration is
a) Anabolic process
b) Exothermic process
c)  Endothermic process
d) Endergonic process

5. The annual plant exchange of gases takes place mainly through
a) Leaf scars
b) lenticels
c) stomata
d) stem

6.  Kreb’s cycle take place in
a) vesicles of ER
b) Mitochondrial matrix
c) lysosomes
d) Dictyosomes

7. Common immediate source of energy in cellular activity:
a) NAD
b) ATP
c)  DNA
d) RNA

8.  Photorespiration involves

Photorespiration involves three organelles:Chloroplast,  Peroxisome and Mitochondria

a) Glycolate cycle
b) kreb’s cycle
c) calvin cycle
d) CAM cycle

9.  In succulent xerophytes the R.Q is :
a) Zero
b) unity
c) less than one
d) more than one

10. R.Q. of fatty substances is generally:
a) unity
b) Zero
c) more than one
d) less than one

11. Conversion of pyruvic acid into ethyl alcohol is facilitated by the enzymes:
a) carboxylase
c) dehygrogenase
d) carboxylase and dehygrogenase

12. During respiration yeast converts glucose to
a) ethanol and water
b) ethanol and oxygen
c) ethanol and CO2
d) lactic acid and CO2

13. The net gain of ATP produced during the oxidation of one glucose molecule in a plant cell
a) 38 ATP molecules
b) 30 ATP molecules
c) 36 ATP molecules
d) 24 ATP molecules

14. The final acceptor of electrons in the electron transport chain is
a) Water
b) Oxygen
c) Hydrogen
d) Cytochrome b

15. In plant cell, digestion of fats occurs with the help of
a) lysosomes
b) Peroxisomes
c) glyoxisomes
d) microtubules

1- d
2- b

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MCQ on Plant Physiology - Photosynthesis

1.  Photosynthetic pigments are located in
a) stroma
b) grana
c) cytoplasm
d) thylakoids
2. The Dark reaction of photosynthesis was worked out by:
a) Hatch and Slack
b) Melvin Clavin
c) Arnold
d) Emerson
3. During photosynthesis oxygen is evolved from
a) carbohydrates
b) proteins
c) sunlight
d) water

4. Light reaction occur in

Chloroplast: Photosynthetic apparatus

a) Grana
b) stroma
c) thylakods
d) mitochondria
5. Photosynthetic pigments absorb
a) UV radiation
b) IR radiation
c) Visible radiation
d) Gama radiation

6.  In cyclic photophorylation the high energy electrons are driven out from
a) Chlorophyll a 683
b) P 700
c) Chlorophyll a 673
d) P 870
7. One among the following elements is very important for photolysis of water
a) Mg
b) Mn
c) Fe
d) Zn
8. The hypothesis that all photosynthesis organisms require a source of hydrogen was first proposed by
a) Van Niel
b) Hatch and Slack
c) Hill
d) Ruber and Kamen
9. In the photosynthesis process, PS II absorbs energy at or just below
a) 700 nm
b) 870 nm
c) 680 nm
d) 780 nm
10. The amount of ATP required for the synthesis of one glucose molecule in C4 pathway is
a) 18 ATP
b) 30 ATP
c) 12 ATP
d) 24 ATP
11.  In bacterial photosynthesis comes from
a) H2S
b) H2O
c) H2SO4
d) NH3
12.  Isotopes popularly known to have been used in study of photosynthesis:
a) C16 and N15
b) C14 and O16
c) P32 and C12
d) C11 and P32
13. Dark reaction is traced by
a) O19
b) P32
c) X-rays
d) 14CO2
14. Photosynthesis is maximum in
a) Blue light
b)  Red light
c)  Blue and green light
d) Blue and red light

15. Law of limiting factor was proposed by
a) Black man
b) Hill
c) Arnold
d) Engle man


  1. d) thylakoids
  2. b) Melvin Clavin
  3. d) water
  4. a) Grana
  5. c) Visible radiation
  6. b) P 700
  7. a) Mg
  8. a) Van Niel
  9. c) 680 nm
  10. b) 30 ATP
  11. a) H2S
  12. b) C14 and O16
  13. d) 14CO2
  14. b)  Red light
  15. a) Black man
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MCQ on Plant Physiology - Mineral Nutrition

 Nitrogen Fixation
Nitrogen Fixation
1. Which of the following is necessary for biological nitrogen fixation?
a) Copper
b) Zinc
c) Manganese
d) Molybdenum

2. The essential element for the synthesis of auxin is
a) Zinc
b) Sulphur
c) Potassium
d) Phosphorus

3. The disease “die back of shoots” is caused due to the deficiency of the following microelements
a) Copper
b) Chlorine
c) Manganese
d) Molybdenum

4. Little leaf disease is due to the deficiency of
a) Nitrogen
b) Zinc
c) Manganese
d) Molybdenum

5. Hydroponics is
a) Growing of aquatic plants
b) Growing off floating aquatic plants
c) Soilless cultivation of plants
d) Growing of plants inside water

6.  Molybdenum is involved in plant metabolism in
a) Translocation of solutes
b) Tryptophan synthesis
c) ABA synthesis
d) Nitrate reduction

7. Plant require Fe and Mg for
a) Synthesis of chlorophyll
b) Opening and closing of stomata
c) Translocation of carbohydrates
d) None of these

8. Plant absorb the element nitrogen in the form
a) nitrogen gas
c) nitrates
d) nitric acid

9. Bacteroid means
a) dead bacteria
b)living bacteria
c) A bacteria like substance
d) living bacteria but cannot divide

10. Which of the following bacterium brings about denitrification?
a) Pseudomonas
b) Rhizobium
c) Azotobacter
d) Nitrobacter

11. Ion can be accumulated against concentration gradient due to?
a) Mass flow
b) Active uptake
c) Passive uptake
d) Donnan equilibrium

12. Which of the following is essential for Nitrogen Fixation by leguminous plants?
a) Chlorophyll
b) Leghaemoglobin
c) Anthocyanin
d) Phycocyanin

13. First experiments related to the method of hydroponics were done by
a) Knop
b) Sachs
c) Arnon
d) Hill

14.  The most widely accepted theory accepted to explain the translocation of carbohydrates in higher plants
a) Root pressure theory
b) Osmotic theory
c) Imbibition theory
d) Mass flow theory

15. Which element is required for Nodulation in Legumes?
a) Mn
b) Fe
c) Mo
d) B


1- d
2- a
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MCQ on Plant Physiology - Plant Water Relations

Translocation of Water
1.  The water readily available to plants for absorption by roots is
a)  Gravitational water
b) Capillary water
c) Rain water
d) Hygroscopic water

2. The water potential of pure water at atmospheric pressure is
a) -2.3 bar
b) +2.3 bar
c) Zero bar
d)  One bar

3. Loss of water from the stomata of leaves are known as
a)  Guttation
b) Exudation
c)  Transpiration
d)  Evaporation

4.  During rainy season wooden doors are difficult to open or closure because of
a) Plasmolysis
b) Imbibition
c) Osmosis
d) Diffusion

5. Plasmolysis occurs due to
a) Absorption
b) Osmosis
c) Endoosmosis
d)  Exosmosis

6.  The marine animals that kept in fresh water burst. It shows the process of
a) Exosmosis
b) Endoosmosis
c) Plasmolysis
d) Deplasmolysis

7. Cooling of plants is caused by
a) Guttaion
b) Photorespiration
c) Transpiration
d) Assimilation

8.  Active uptake of minerals by roots mainly depends on the
a)  Availability of oxygen
b) Temperature
c) Light
d) Availability of CO2

9.  The hormone which signals the closure of stomata is
a) Auxins
b) Cytokinine
c) Gibberelline
d)  Abscisic acid

10.  Water absorption takes place through
a) Lateral roots
b) Root cap
c) Root hairs
d) Tap root

11. Which of the following is an anti-transpirant
a) PMA
b) PAN
c) IAA
d) AUG

12. What is the action spectrum of transpiration?
a) Orange and red
b) Green and ultraviolet
c) Blue and red
d) None of these

13. Which one of the following is used for measuring the rate of transpiration?
a) Porometer
b) Osmometer
c) Moll’s experiment
d) Potometer

14. Transpiration is least in
a) High atmospheric humidity
b) good soil moisture
c) high wind velocity
d) dry environment

15. Stomata open at night and close during day time in
a) Xerophytes
b) Mesophytes
c) Succulents
d) Hydrophytes

1- b
2- c

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